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{{STRUCTURE_3a6m|  PDB=3a6m  |  SCENE=  }}
===Crystal structure of GrpE from Thermus thermophilus HB8===
{{ABSTRACT_PUBMED_20036249}}


==Function==
==Crystal structure of GrpE from Thermus thermophilus HB8==
[[http://www.uniprot.org/uniprot/GRPE_THET8 GRPE_THET8]] Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding (By similarity).  
<StructureSection load='3a6m' size='340' side='right'caption='[[3a6m]], [[Resolution|resolution]] 3.23&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[3a6m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3A6M FirstGlance]. <br>
[[3a6m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3A6M OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.23&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3a6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3a6m OCA], [https://pdbe.org/3a6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3a6m RCSB], [https://www.ebi.ac.uk/pdbsum/3a6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3a6m ProSAT]</span></td></tr>
==Reference==
</table>
<ref group="xtra">PMID:020036249</ref><references group="xtra"/><references/>
== Function ==
[[Category: Thet8]]
[https://www.uniprot.org/uniprot/GRPE_THET8 GRPE_THET8] Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with DnaK and GrpE. It is the nucleotide exchange factor for DnaK and may function as a thermosensor. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding (By similarity).
[[Category: Miki, K.]]
== Evolutionary Conservation ==
[[Category: Nakamura, A.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Takumi, K.]]
Check<jmol>
[[Category: Chaperone]]
  <jmolCheckbox>
[[Category: Coiled-coil]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a6/3a6m_consurf.spt"</scriptWhenChecked>
[[Category: Dimer]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: Four-helix bundle]]
    <text>to colour the structure by Evolutionary Conservation</text>
[[Category: Grpe]]
  </jmolCheckbox>
[[Category: Stress response]]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3a6m ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermus thermophilus HB8]]
[[Category: Miki K]]
[[Category: Nakamura A]]
[[Category: Takumi K]]
Retrieved from "https://proteopedia.org/3a6m"