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{{STRUCTURE_3w43|  PDB=3w43  |  SCENE=  }}
===Crystal structure of RsbX in complex with manganese in space group P21===


==Function==
==Crystal structure of RsbX in complex with manganese in space group P21==
[[http://www.uniprot.org/uniprot/RSBX_BACSU RSBX_BACSU]] Negative regulator of sigma-B activity. Dephosphorylates RsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communicating sigma-B protein levels.<ref>PMID:1592822</ref> <ref>PMID:8468294</ref> <ref>PMID:8824586</ref> <ref>PMID:9658013</ref>
<StructureSection load='3w43' size='340' side='right'caption='[[3w43]], [[Resolution|resolution]] 1.22&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3w43]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W43 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3W43 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.22&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3w43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3w43 OCA], [https://pdbe.org/3w43 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3w43 RCSB], [https://www.ebi.ac.uk/pdbsum/3w43 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3w43 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RSBX_BACSU RSBX_BACSU] Negative regulator of sigma-B activity. Dephosphorylates RsbS. Plays a role both in maintaining low sigma-B activity during growth and in reestablishing prestress sigma-B activity after induction. Could have a negative feedback role by indirectly communicating sigma-B protein levels.<ref>PMID:1592822</ref> <ref>PMID:8468294</ref> <ref>PMID:8824586</ref> <ref>PMID:9658013</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the general stress response of Bacillus subtilis, which is governed by the sigma factor sigma(B), stress signalling is relayed by a cascade of Rsb proteins that regulate sigma(B) activity. RsbX, a PPM II phosphatase, halts the response by dephosphorylating the stressosome composed of RsbR and RsbS. The crystal structure of RsbX reveals a reorganization of the catalytic centre, with the second Mn(2+) ion uniquely coordinated by Gly47 O from the beta4-alpha1 loop instead of a water molecule as in PPM I phosphatases. An extra helical turn of alpha1 tilts the loop towards the metal-binding site, and the beta2-beta3 loop swings outwards to accommodate this tilting. The residues critical for this defining feature of the PPM II phosphatases are highly conserved. Formation of the catalytic centre is metal-specific, as crystallization with Mg(2+) ions resulted in a shift of the beta4-alpha1 loop that led to loss of the second ion. RsbX also lacks the flap subdomain characteristic of PPM I phosphatases. On the basis of a stressosome model, the activity of RsbX towards RsbR-P and RsbS-P may be influenced by the different accessibilities of their phosphorylation sites.


==About this Structure==
Structure of the RsbX phosphatase involved in the general stress response of Bacillus subtilis.,Teh AH, Makino M, Hoshino T, Baba S, Shimizu N, Yamamoto M, Kumasaka T Acta Crystallogr D Biol Crystallogr. 2015 Jun;71(Pt 6):1392-9. doi:, 10.1107/S1399004715007166. Epub 2015 May 23. PMID:26057679<ref>PMID:26057679</ref>
[[3w43]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3W43 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<references group="xtra"/><references/>
</div>
[[Category: Phosphoserine phosphatase]]
<div class="pdbe-citations 3w43" style="background-color:#fffaf0;"></div>
[[Category: Baba, S.]]
 
[[Category: Kumasaka, T.]]
==See Also==
[[Category: Makino, M.]]
*[[Phosphoserine phosphatase|Phosphoserine phosphatase]]
[[Category: Shimizu, N.]]
== References ==
[[Category: Teh, A H.]]
<references/>
[[Category: Yamamoto, M.]]
__TOC__
[[Category: Alpha-beta beta-alpha sandwich fold]]
</StructureSection>
[[Category: Dephosphorylation]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Environmental stress]]
[[Category: Large Structures]]
[[Category: Hydrolase]]
[[Category: Baba S]]
[[Category: Magnesium/manganese binding]]
[[Category: Kumasaka T]]
[[Category: Phosphatase]]
[[Category: Makino M]]
[[Category: Phosphoric monoester hydrolase]]
[[Category: Shimizu N]]
[[Category: Protein phosphatase]]
[[Category: Teh AH]]
[[Category: Signaling protein]]
[[Category: Yamamoto M]]
[[Category: Stressosome]]
[[Category: Tertiary]]
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