300d: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(12 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:300d.jpg|left|200px]]<br /><applet load="300d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="300d, resolution 3.000&Aring;" />
'''CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MN(II)-SOAKED'''<br />


==Overview==
==CAPTURING THE STRUCTURE OF A CATALYTIC RNA INTERMEDIATE: RNA HAMMERHEAD RIBOZYME, MN(II)-SOAKED==
The crystal structure of an unmodified hammerhead RNA in the absence of divalent metal ions has been solved, and it was shown that this ribozyme can cleave itself in the crystal when divalent metal ions are added. This biologically active RNA fold is the same as that found previously for two modified hammerhead ribozymes. Addition of divalent cations at low pH makes it possible to capture the uncleaved RNA in metal-bound form. A conformational intermediate, having an additional Mg(II) bound to the cleavage-site phosphate, was captured by freeze-trapping the RNA at an active pH prior to cleavage. The most significant conformational changes were limited to the active site of the ribozyme, and the changed conformation requires only small additional movements to reach a proposed transition-state.
<StructureSection load='300d' size='340' side='right'caption='[[300d]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[300d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=300D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=300D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=300d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=300d OCA], [https://pdbe.org/300d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=300d RCSB], [https://www.ebi.ac.uk/pdbsum/300d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=300d ProSAT]</span></td></tr>
</table>


==About this Structure==
==See Also==
300D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=300D OCA].
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Capturing the structure of a catalytic RNA intermediate: the hammerhead ribozyme., Scott WG, Murray JB, Arnold JR, Stoddard BL, Klug A, Science. 1996 Dec 20;274(5295):2065-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8953035 8953035]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Arnold JRP]]
[[Category: Arnold, J R.P.]]
[[Category: Klug A]]
[[Category: Klug, A.]]
[[Category: Murray JB]]
[[Category: Murray, J B.]]
[[Category: Scott WG]]
[[Category: Scott, W G.]]
[[Category: Stoddard BL]]
[[Category: Stoddard, B L.]]
[[Category: MN]]
[[Category: catalytic rna]]
[[Category: loop]]
[[Category: rna hammerhead ribozyme]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:01:46 2008''
Retrieved from "https://proteopedia.org/300d"