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| [[Image:333d.gif|left|200px]]<br /><applet load="333d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="333d, resolution 2.520Å" />
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| '''THE CRYSTAL STRUCTURE OF AN RNA OLIGOMER INCORPORATING TANDEM ADENOSINE-INOSINE MISMATCHES'''<br />
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| ==Overview== | | ==THE CRYSTAL STRUCTURE OF AN RNA OLIGOMER INCORPORATING TANDEM ADENOSINE-INOSINE MISMATCHES== |
| The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has been refined to 2.5 A. It shows a symmetric internal loop of two non-Watson-Crick base pairs which form in the middle of the duplex. The tandem A-I/I-A pairs are related by a crystallographic two-fold axis. Both A(anti)-I(anti) mismatches are in a head-to-head conformation forming hydrogen bonds using the Watson-Crick positions. The octamer duplexes stack above one another in the cell forming a pseudo-infinite helix throughout the crystal. A hydrated calcium ion bridges between the 3'-terminal of one molecule and the backbone of another. The tandem A-I mismatches are incorporated with only minor distortion to the backbone. This is in contrast to the large helical perturbations often produced by sheared G-A pairs in RNA oligonucleotides.
| | <StructureSection load='333d' size='340' side='right'caption='[[333d]], [[Resolution|resolution]] 2.52Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[333d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=333D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=333D FirstGlance]. <br> |
| 333D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=333D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52Å</td></tr> |
| | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| ==Reference==
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=333d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=333d OCA], [https://pdbe.org/333d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=333d RCSB], [https://www.ebi.ac.uk/pdbsum/333d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=333d ProSAT]</span></td></tr> |
| The crystal structure of an RNA oligomer incorporating tandem adenosine-inosine mismatches., Carter RJ, Baeyens KJ, SantaLucia J, Turner DH, Holbrook SR, Nucleic Acids Res. 1997 Oct 15;25(20):4117-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9321667 9321667]
| | </table> |
| [[Category: Protein complex]] | | __TOC__ |
| [[Category: Baeyens, K J.]]
| | </StructureSection> |
| [[Category: Carter, R J.]]
| | [[Category: Large Structures]] |
| [[Category: Holbrook, S R.]]
| | [[Category: Baeyens KJ]] |
| [[Category: Jr., J SantaLucia.]] | | [[Category: Carter RJ]] |
| [[Category: Turner, D H.]] | | [[Category: Holbrook SR]] |
| [[Category: CA]] | | [[Category: SantaLucia Jr J]] |
| [[Category: a-rna]] | | [[Category: Turner DH]] |
| [[Category: double helix]] | |
| [[Category: internal loop]] | |
| [[Category: mismatched]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:05 2008''
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