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| [[Image:330d.gif|left|200px]]<br /><applet load="330d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="330d, resolution 2.700Å" />
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| '''BASE-PAIRING SHIFT IN THE MAJOR GROOVE OF (CA)N TRACTS BY B-DNA CRYSTAL STRUCTURES'''<br />
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| ==Overview== | | ==BASE-PAIRING SHIFT IN THE MAJOR GROOVE OF (CA)N TRACTS BY B-DNA CRYSTAL STRUCTURES== |
| the crystal packing of the B-DNA dodecamer d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of double helices with specific base-backbone interactions in the major groove. Cooling the crystals below -10 degrees C stabilizes a new conformational state with a long-range sequence-dependent one-step shift in the major-groove base pairing. The tilt of the bases leads to the disruption of the Watson-Crick pairing in the major groove and to the formation of interactions with the 5' neighbour of their complement. This alteration propagates along the helical axis over more than half a turn. As a result, the molecular structure is normal when seen from the minor groove side and mismatched in the major groove. Comparison with a parent isomorphous dodecamer structure corresponding to the codon 10-13 of the c-Ha-ras proto-oncogene show that this new structural feature is sequence dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are involved both in recombination and in transcription, this new recognition pattern should be considered in the analysis of the various processes involving the reading of the genetic information.
| | <StructureSection load='330d' size='340' side='right'caption='[[330d]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[330d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=330D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=330D FirstGlance]. <br> |
| 330D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=330D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=330d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=330d OCA], [https://pdbe.org/330d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=330d RCSB], [https://www.ebi.ac.uk/pdbsum/330d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=330d ProSAT]</span></td></tr> |
| ==Reference== | | </table> |
| Base-pairing shift in the major groove of (CA)n tracts by B-DNA crystal structures., Timsit Y, Vilbois E, Moras D, Nature. 1991 Nov 14;354(6349):167-70. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1944598 1944598]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Moras, D.]] | | [[Category: Large Structures]] |
| [[Category: Timsit, Y.]] | | [[Category: Moras D]] |
| [[Category: Vilbois, E.]] | | [[Category: Timsit Y]] |
| [[Category: b-dna]] | | [[Category: Vilbois E]] |
| [[Category: double helix]] | |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:02:05 2008''
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