4oku: Difference between revisions
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==Structure of Toxoplasma gondii proMIC2== | |||
<StructureSection load='4oku' size='340' side='right'caption='[[4oku]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4oku]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OKU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oku FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oku OCA], [https://pdbe.org/4oku PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oku RCSB], [https://www.ebi.ac.uk/pdbsum/4oku PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oku ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O00816_TOXGO O00816_TOXGO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Micronemal protein 2 (MIC2) is the key adhesin that supports gliding motility and host cell invasion by Toxoplasma gondii. With a von Willebrand factor A (VWA) domain and six thrombospondin repeat domains (TSR1-6) in its ectodomain, MIC2 connects to the parasite actomyosin system through its cytoplasmic tail. MIC2-associated protein (M2AP) binds noncovalently to the MIC2 ectodomain. MIC2 and M2AP are stored in micronemes as proforms. We find that the MIC2-M2AP ectodomain complex is a highly elongated 1:1 monomer with M2AP bound to the TSR6 domain. Crystal structures of N-terminal fragments containing the VWA and TSR1 domains for proMIC2 and MIC2 reveal a closed conformation of the VWA domain and how it associates with the TSR1 domain. A long, proline-rich, disulfide-bonded pigtail loop in TSR1 overlaps the VWA domain. Mannose alpha-C-linked to Trp-276 in TSR1 has an unusual (1)C4 chair conformation. The MIC2 VWA domain includes a mobile alpha5-helix and a 22-residue disordered region containing two disulfide bonds in place of an alpha6-helix. A hydrophobic residue in the prodomain binds to a pocket adjacent to the alpha7-helix that pistons in opening of the VWA domain to a putative high-affinity state. | |||
Structures of the Toxoplasma gliding motility adhesin.,Song G, Springer TA Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):4862-7. doi:, 10.1073/pnas.1403059111. Epub 2014 Mar 17. PMID:24639528<ref>PMID:24639528</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4oku" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Toxoplasma gondii]] | |||
[[Category: Song G]] | |||
[[Category: Springer TA]] | |||
Latest revision as of 10:22, 30 October 2024
Structure of Toxoplasma gondii proMIC2
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