2iuo: Difference between revisions

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{{STRUCTURE_2iuo|  PDB=2iuo  |  SCENE=  }}
===Site Directed Mutagenesis of Key Residues Involved in the Catalytic Mechnism of Cyanase===
{{ABSTRACT_PUBMED_10801492}}


==Function==
==Site Directed Mutagenesis of Key Residues Involved in the Catalytic Mechanism of Cyanase==
[[http://www.uniprot.org/uniprot/CYNS_ECOLI CYNS_ECOLI]] Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.  
<StructureSection load='2iuo' size='340' side='right'caption='[[2iuo]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[2iuo]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IUO FirstGlance]. <br>
[[2iuo]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IUO OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iuo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iuo OCA], [https://pdbe.org/2iuo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iuo RCSB], [https://www.ebi.ac.uk/pdbsum/2iuo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iuo ProSAT]</span></td></tr>
<ref group="xtra">PMID:010801492</ref><references group="xtra"/><references/>
</table>
[[Category: Bacillus coli migula 1895]]
== Function ==
[[Category: Cyanase]]
[https://www.uniprot.org/uniprot/CYNS_ECOLI CYNS_ECOLI] Catalyzes the reaction of cyanate with bicarbonate to produce ammonia and carbon dioxide.
[[Category: Anderson, M P.]]
== Evolutionary Conservation ==
[[Category: Guilloton, M.]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: Joachimiak, A.]]
Check<jmol>
[[Category: Walsh, M A.]]
  <jmolCheckbox>
[[Category: Cyanate degradation]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iuo_consurf.spt"</scriptWhenChecked>
[[Category: Lyase]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iuo ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Anderson MP]]
[[Category: Guilloton M]]
[[Category: Joachimiak A]]
[[Category: Walsh MA]]