4ol8: Difference between revisions
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==Ty3 reverse transcriptase bound to DNA/RNA== | |||
<StructureSection load='4ol8' size='340' side='right'caption='[[4ol8]], [[Resolution|resolution]] 3.10Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ol8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OL8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ol8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ol8 OCA], [https://pdbe.org/4ol8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ol8 RCSB], [https://www.ebi.ac.uk/pdbsum/4ol8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ol8 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Retrotransposons are a class of mobile genetic elements that replicate by converting their single-stranded RNA intermediate to double-stranded DNA through the combined DNA polymerase and ribonuclease H (RNase H) activities of the element-encoded reverse transcriptase (RT). Although a wealth of structural information is available for lentiviral and gammaretroviral RTs, equivalent studies on counterpart enzymes of long terminal repeat (LTR)-containing retrotransposons, from which they are evolutionarily derived, is lacking. In this study, we report the first crystal structure of a complex of RT from the Saccharomyces cerevisiae LTR retrotransposon Ty3 in the presence of its polypurine tract-containing RNA-DNA hybrid. In contrast to its retroviral counterparts, Ty3 RT adopts an asymmetric homodimeric architecture whose assembly is substrate dependent. Moreover, our structure and biochemical data suggest that the RNase H and DNA polymerase activities are contributed by individual subunits of the homodimer. | |||
Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry.,Nowak E, Miller JT, Bona MK, Studnicka J, Szczepanowski RH, Jurkowski J, Le Grice SF, Nowotny M Nat Struct Mol Biol. 2014 Mar 9. doi: 10.1038/nsmb.2785. PMID:24608367<ref>PMID:24608367</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4ol8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Reverse transcriptase 3D structures|Reverse transcriptase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharomyces cerevisiae]] | |||
[[Category: Bona MK]] | |||
[[Category: Jurkowski J]] | |||
[[Category: Le Grice SFJ]] | |||
[[Category: Miller JT]] | |||
[[Category: Nowak E]] | |||
[[Category: Nowotny M]] | |||
[[Category: Studnicka J]] | |||
[[Category: Szczepanowski RH]] | |||
Latest revision as of 03:22, 21 November 2024
Ty3 reverse transcriptase bound to DNA/RNA
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