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{{STRUCTURE_4g7v|  PDB=4g7v  |  SCENE=  }}
===Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.5 A)===


==About this Structure==
==Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.5 A)==
[[4g7v]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7V OCA].  
<StructureSection load='4g7v' size='340' side='right'caption='[[4g7v]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
[[Category: Li, Q.]]
== Structural highlights ==
[[Category: Alpha helix]]
<table><tr><td colspan='2'>[[4g7v]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ciona_intestinalis Ciona intestinalis] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4G7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4G7V FirstGlance]. <br>
[[Category: Fragment antibody]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
[[Category: Membrane protein]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
[[Category: Sensing voltage]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4g7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4g7v OCA], [https://pdbe.org/4g7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4g7v RCSB], [https://www.ebi.ac.uk/pdbsum/4g7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4g7v ProSAT]</span></td></tr>
[[Category: Voltage sensing domain]]
</table>
== Function ==
[https://www.uniprot.org/uniprot/F6XHE4_CIOIN F6XHE4_CIOIN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The transduction of transmembrane electric fields into protein motion has an essential role in the generation and propagation of cellular signals. Voltage-sensing domains (VSDs) carry out these functions through reorientations of positive charges in the S4 helix. Here, we determined crystal structures of the Ciona intestinalis VSD (Ci-VSD) in putatively active and resting conformations. S4 undergoes an ~5-A displacement along its main axis, accompanied by an ~60 degrees rotation. This movement is stabilized by an exchange in countercharge partners in helices S1 and S3 that generates an estimated net charge transfer of ~1 eo. Gating charges move relative to a ''hydrophobic gasket' that electrically divides intra- and extracellular compartments. EPR spectroscopy confirms the limited nature of S4 movement in a membrane environment. These results provide an explicit mechanism for voltage sensing and set the basis for electromechanical coupling in voltage-dependent enzymes and ion channels.
 
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.,Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E Nat Struct Mol Biol. 2014 Mar;21(3):244-52. doi: 10.1038/nsmb.2768. Epub 2014 Feb, 2. PMID:24487958<ref>PMID:24487958</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4g7v" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[3D structures of human antibody|3D structures of human antibody]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Ciona intestinalis]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Li Q]]
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