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{{STRUCTURE_4gla|  PDB=4gla  |  SCENE=  }}
===OBody NL8 bound to hen egg-white lysozyme===
{{ABSTRACT_PUBMED_24465865}}


==Function==
==OBody NL8 bound to hen egg-white lysozyme==
[[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
<StructureSection load='4gla' size='340' side='right'caption='[[4gla]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4gla]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Pyrobaculum_aerophilum Pyrobaculum aerophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GLA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gla OCA], [https://pdbe.org/4gla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gla RCSB], [https://www.ebi.ac.uk/pdbsum/4gla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gla ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The OB-fold is a small, versatile single-domain protein binding module that occurs in all forms of life, where it binds protein, carbohydrate, nucleic acid and small-molecule ligands. We have exploited this natural plasticity to engineer a new class of non-immunoglobulin alternatives to antibodies with unique structural and biophysical characteristics. We present here the engineering of the OB-fold anticodon recognition domain from aspartyl tRNA synthetase taken from the thermophile Pyrobaculum aerophilum. For this single-domain scaffold we have coined the term OBody. Starting from a naive combinatorial library, we engineered an OBody with 3 nM affinity for hen egg-white lysozyme, by optimising the affinity of a naive OBody 11,700-fold over several affinity maturation steps, using phage display. At each maturation step a crystal structure of the engineered OBody in complex with hen egg-white lysozyme was determined, showing binding elements in atomic detail. These structures have given us an unprecedented insight into the directed evolution of affinity for a single antigen on the molecular scale. The engineered OBodies retain the high thermal stability of the parental OB-fold despite mutation of up to 22% of their residues. They can be expressed in soluble form and also purified from bacteria at high yields. They also lack disulfide bonds. These data demonstrate the potential of OBodies as a new scaffold for the engineering of specific binding reagents and provide a platform for further development of future OBody-based applications.


==About this Structure==
Tracking Molecular Recognition at the Atomic Level with a New Protein Scaffold Based on the OB-Fold.,Steemson JD, Baake M, Rakonjac J, Arcus VL, Liddament MT PLoS One. 2014 Jan 20;9(1):e86050. doi: 10.1371/journal.pone.0086050. eCollection, 2014 Jan 20. PMID:24465865<ref>PMID:24465865</ref>
[[4gla]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_51768 Atcc 51768] and [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GLA OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<ref group="xtra">PMID:024465865</ref><references group="xtra"/><references/>
</div>
[[Category: Atcc 51768]]
<div class="pdbe-citations 4gla" style="background-color:#fffaf0;"></div>
[[Category: Chick]]
 
[[Category: Lysozyme]]
==See Also==
[[Category: Steemson, J D.]]
*[[Lysozyme 3D structures|Lysozyme 3D structures]]
[[Category: Beta barrel]]
== References ==
[[Category: Engineered binding protein]]
<references/>
[[Category: Enzyme inhibition]]
__TOC__
[[Category: Hydrolase-de novo protein complex]]
</StructureSection>
[[Category: Muraminidase]]
[[Category: Gallus gallus]]
[[Category: Novel scaffold]]
[[Category: Large Structures]]
[[Category: Ob-fold]]
[[Category: Pyrobaculum aerophilum]]
[[Category: Protein-protein complex]]
[[Category: Steemson JD]]
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