4co6: Difference between revisions

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~~'''Unreleased structure'''~~

The ~~entry 4co6~~ is ~~ON HOLD until Paper~~ Publication

==Crystal structure of the Nipah virus RNA free nucleoprotein- phosphoprotein complex==

== Structural highlights ==

<table><tr><td colspan='2'>[[4co6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Henipavirus_nipahense Henipavirus nipahense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CO6 FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.498Å</td></tr>

<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4co6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4co6 OCA], [https://pdbe.org/4co6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4co6 RCSB], [https://www.ebi.ac.uk/pdbsum/4co6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4co6 ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/NCAP_NIPAV NCAP_NIPAV] Encapsidates the genome protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (By similarity).

== Publication Abstract from PubMed ==

Nipah virus (NiV) is a highly pathogenic emergent paramyxovirus causing deadly encephalitis in humans. Its replication requires a constant supply of unassembled nucleoprotein (N0) in complex with its viral chaperone, the phosphoprotein (P). To elucidate the chaperone function of P, we reconstituted NiV the N0-P core complex and determined its crystal structure. The binding of the N-terminal region of P blocks the polymerization of N by interfering with subdomain exchange between N protomers and keeps N0 in an open conformation, ready to grasp an RNA molecule. We found that a peptide derived from the N-binding region of P protects cells against viral infection and demonstrated by structure-based mutagenesis that this peptide acts by inhibiting N0-P formation. These results provide new insights about the assembly of N along genomic RNA and validate the N0-P complex as a target for drug development.

~~Authors: Yabukarksi~~, F., Lawrence, P., Tarbouriech, N., Bourhis, ~~J.M.~~, Jensen~~, M.R.~~, Ruigrok~~, R.W.H.~~, Blackledge, M., Volchkov, V., Jamin, M.

Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone.,Yabukarski F, Lawrence P, Tarbouriech N, Bourhis JM, Delaforge E, Jensen MR, Ruigrok RW, Blackledge M, Volchkov V, Jamin M Nat Struct Mol Biol. 2014 Aug 10. doi: 10.1038/nsmb.2868. PMID:25108352<ref>PMID:25108352</ref>

~~Description: Crystal structure~~ of the ~~Nipah virus RNA free nucleoprotein~~-~~phosphoprotein complex~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

==See Also==

*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]

== References ==

__TOC__

</StructureSection>

[[Category: Henipavirus nipahense]]

[[Category: Large Structures]]

[[Category: Blackledge M]]

[[Category: Bourhis JM]]

[[Category: Jamin M]]

[[Category: Jensen MR]]

[[Category: Lawrence P]]

[[Category: Ruigrok RWH]]

[[Category: Tarbouriech N]]

[[Category: Volchkov V]]

[[Category: Yabukarksi F]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 4co6 is ON HOLD  until Paper Publication
+==Crystal structure of the Nipah virus RNA free nucleoprotein- phosphoprotein complex==
+<StructureSection load='4co6' size='340' side='right'caption='[[4co6]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4co6]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Henipavirus_nipahense Henipavirus nipahense]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CO6 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.498&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4co6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4co6 OCA], [https://pdbe.org/4co6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4co6 RCSB], [https://www.ebi.ac.uk/pdbsum/4co6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4co6 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NCAP_NIPAV NCAP_NIPAV] Encapsidates the genome protecting it from nucleases. The encapsidated genomic RNA is termed the nucleocapsid (NC) and serves as template for transcription and replication (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Nipah virus (NiV) is a highly pathogenic emergent paramyxovirus causing deadly encephalitis in humans. Its replication requires a constant supply of unassembled nucleoprotein (N0) in complex with its viral chaperone, the phosphoprotein (P). To elucidate the chaperone function of P, we reconstituted NiV the N0-P core complex and determined its crystal structure. The binding of the N-terminal region of P blocks the polymerization of N by interfering with subdomain exchange between N protomers and keeps N0 in an open conformation, ready to grasp an RNA molecule. We found that a peptide derived from the N-binding region of P protects cells against viral infection and demonstrated by structure-based mutagenesis that this peptide acts by inhibiting N0-P formation. These results provide new insights about the assembly of N along genomic RNA and validate the N0-P complex as a target for drug development.
-Authors: Yabukarksi, F., Lawrence, P., Tarbouriech, N., Bourhis, J.M., Jensen, M.R., Ruigrok, R.W.H., Blackledge, M., Volchkov, V., Jamin, M.
+Structure of Nipah virus unassembled nucleoprotein in complex with its viral chaperone.,Yabukarski F, Lawrence P, Tarbouriech N, Bourhis JM, Delaforge E, Jensen MR, Ruigrok RW, Blackledge M, Volchkov V, Jamin M Nat Struct Mol Biol. 2014 Aug 10. doi: 10.1038/nsmb.2868. PMID:25108352<ref>PMID:25108352</ref>
-Description: Crystal structure of the Nipah virus RNA free nucleoprotein-phosphoprotein complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 4co6" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Henipavirus nipahense]]
+[[Category: Large Structures]]
+[[Category: Blackledge M]]
+[[Category: Bourhis JM]]
+[[Category: Jamin M]]
+[[Category: Jensen MR]]
+[[Category: Lawrence P]]
+[[Category: Ruigrok RWH]]
+[[Category: Tarbouriech N]]
+[[Category: Volchkov V]]
+[[Category: Yabukarksi F]]