2bp6: Difference between revisions

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[[Image:2bp6.gif|left|200px]]<br />
<applet load="2bp6" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2bp6, resolution 1.50&Aring;" />
'''CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN (PA-IIL) COMPLEXED WITH A-L-GALACTOPYRANOSIDE'''<br />


==Overview==
==crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with a-L-Galactopyranoside==
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host, recognition and biofilm formation. Lectin not only displays an unusually, high affinity for fucose but also binds to L-fucose, L-galactose and, D-arabinose that differ only by the group at position 5 of the sugar ring., Isothermal calorimetry experiments provided precise determination of, affinity for the three methyl-glycosides and revealed a large enthalpy, contribution. The crystal structures of the complexes of PA-IIL with, L-galactose and Met-beta-D-arabinoside have been determined and compared, with the PA-IIL/fucose complex described previously. A combination of the, structures and thermodynamics provided clues for the role of the, hydrophobic group in affinity.
<StructureSection load='2bp6' size='340' side='right'caption='[[2bp6]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bp6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BP6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BP6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GXL:ALPHA-L-GALACTOPYRANOSE'>GXL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bp6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bp6 OCA], [https://pdbe.org/2bp6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bp6 RCSB], [https://www.ebi.ac.uk/pdbsum/2bp6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bp6 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9HYN5_PSEAE Q9HYN5_PSEAE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bp/2bp6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bp6 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The lectin from Pseudomonas aeruginosa (PA-IIL) is involved in host recognition and biofilm formation. Lectin not only displays an unusually high affinity for fucose but also binds to L-fucose, L-galactose and D-arabinose that differ only by the group at position 5 of the sugar ring. Isothermal calorimetry experiments provided precise determination of affinity for the three methyl-glycosides and revealed a large enthalpy contribution. The crystal structures of the complexes of PA-IIL with L-galactose and Met-beta-D-arabinoside have been determined and compared with the PA-IIL/fucose complex described previously. A combination of the structures and thermodynamics provided clues for the role of the hydrophobic group in affinity.


==About this Structure==
Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures.,Sabin C, Mitchell EP, Pokorna M, Gautier C, Utille JP, Wimmerova M, Imberty A FEBS Lett. 2006 Feb 6;580(3):982-7. Epub 2006 Jan 19. PMID:16438968<ref>PMID:16438968</ref>
2BP6 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with GXL, CA and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BP6 OCA]].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Binding of different monosaccharides by lectin PA-IIL from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures., Sabin C, Mitchell EP, Pokorna M, Gautier C, Utille JP, Wimmerova M, Imberty A, FEBS Lett. 2006 Feb 6;580(3):982-7. Epub 2006 Jan 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16438968 16438968]
</div>
[[Category: Pseudomonas aeruginosa]]
<div class="pdbe-citations 2bp6" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Imberty, A.]]
<references/>
[[Category: Mitchell, E.P.]]
__TOC__
[[Category: Sabin, C.D.]]
</StructureSection>
[[Category: Wimmerova, M.]]
[[Category: Large Structures]]
[[Category: CA]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: GXL]]
[[Category: Imberty A]]
[[Category: SO4]]
[[Category: Mitchell EP]]
[[Category: calcium]]
[[Category: Sabin CD]]
[[Category: galactose]]
[[Category: Wimmerova M]]
[[Category: lectin]]
[[Category: pseudomonas aeruginosa]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 16:44:30 2007''

Latest revision as of 13:51, 13 December 2023

crystal Structure of pseudomonas aeruginosa lectin (PA-IIL) complexed with a-L-Galactopyranoside

2bp6, resolution 1.50Å

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