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[[Image:3lkf.jpg|left|200px]]<br /><applet load="3lkf" size="350" color="white" frame="true" align="right" spinBox="true"
caption="3lkf, resolution 1.90&Aring;" />
'''LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS WITH PHOSPHOCHOLINE BOUND'''<br />


==Overview==
==LEUKOCIDIN F (HLGB) FROM STAPHYLOCOCCUS AUREUS WITH PHOSPHOCHOLINE BOUND==
Staphylococcal LukF, LukS, HgammaII, and alpha-hemolysin are self-assembling, channel-forming proteins related in sequence and function. In the alpha-hemolysin heptamer, the channel-forming beta-strands and the amino latch make long excursions from the protomer core. Here we report the crystal structure of the water soluble form of LukF. In the LukF structure the channel-forming region folds into an amphipathic, three-strand beta-sheet and the amino latch forms a beta-strand extending a central beta-sheet. The LukF structure illustrates how a channel-forming toxin masks protein-protein and protein-membrane interfaces prior to cell binding and assembly, and together with the alpha-hemolysin heptamer structure, they define the end points on the pathway of toxin assembly.
<StructureSection load='3lkf' size='340' side='right'caption='[[3lkf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3lkf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LKF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PC:PHOSPHOCHOLINE'>PC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lkf OCA], [https://pdbe.org/3lkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lkf RCSB], [https://www.ebi.ac.uk/pdbsum/3lkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lkf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HLGB_STAAU HLGB_STAAU] Toxin that seems to act by forming pores in the membrane of the cell. Has a hemolytic and a leucotoxic activity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lk/3lkf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lkf ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
3LKF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=PC:'>PC</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LKF OCA].
*[[Pore forming toxin%2C α-hemolysin|Pore forming toxin%2C α-hemolysin]]
 
__TOC__
==Reference==
</StructureSection>
Crystal structure of staphylococcal LukF delineates conformational changes accompanying formation of a transmembrane channel., Olson R, Nariya H, Yokota K, Kamio Y, Gouaux E, Nat Struct Biol. 1999 Feb;6(2):134-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10048924 10048924]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Staphylococcus aureus]]
[[Category: Staphylococcus aureus]]
[[Category: Gouaux, J E.]]
[[Category: Gouaux JE]]
[[Category: Kamio, Y.]]
[[Category: Kamio Y]]
[[Category: Nariya, H.]]
[[Category: Nariya H]]
[[Category: Olson, R.]]
[[Category: Olson R]]
[[Category: Yokota, K.]]
[[Category: Yokota K]]
[[Category: PC]]
[[Category: hemolysin]]
[[Category: leukotoxin]]
[[Category: pore-forming toxin]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:10:04 2008''
Retrieved from "https://proteopedia.org/3lkf"