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| [[Image:405d.gif|left|200px]]<br /><applet load="405d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="405d, resolution 2.50Å" />
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| '''STRUCTURE OF A 16-MER RNA DUPLEX R(GCAGACUUAAAUCUGC)2 WITH WOBBLE LIKE A.C MISMATCHES'''<br />
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| ==Overview== | | ==STRUCTURE OF A 16-MER RNA DUPLEX R(GCAGACUUAAAUCUGC)2 WITH WOBBLE LIKE A.C MISMATCHES== |
| The crystal structure of a 16-mer, the longest known RNA duplex, has been determined at 2.5 A resolution. The hexadecamer r(GCAGACUUAAAUCUGC) contains isolated C.A/A.C mismatches with two hydrogen bonds. The two hydrogen bonds in the mismatches suggests that N1 of A is protonated even though the crystallization was done at neutral pH. Therefore, the C.A mismatch is a C.A+ wobble similar to the G.U wobble. The two C.A+ pairs are isolated by four Watson-Crick pairs and flanked by five Watson-Crick base-pairs on either sides. Kinks/bends of 20 degrees are observed at the wobble sites. The Watson-Crick base-pair A5.U26 on the 5'-side of the first C6.A27(+) wobble has a twist angle of 27 degrees compared to the 3'-side U7.A28 pair of 36 degrees. The twist angles are reversed (37 degrees and 26 degrees) in the second A11(+).C22 wobble because of the approximate dyad in the molecule, the flanking base-pair sequences are A.U pairs. The wobbles expand the major groove to 7.1 A/7.3 A. The duplexes form helical columns and are tightly packed around the 31-screw axis. The minor grooves of adjacent columns in juxtaposition interact through the O2' atoms and the anionic phosphate oxygen atoms.Copyright 1998 Academic Press.
| | <StructureSection load='405d' size='340' side='right'caption='[[405d]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[405d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=405D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=405D FirstGlance]. <br> |
| 405D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=405D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=405d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=405d OCA], [https://pdbe.org/405d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=405d RCSB], [https://www.ebi.ac.uk/pdbsum/405d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=405d ProSAT]</span></td></tr> |
| ==Reference==
| | </table> |
| Structure of a 16-mer RNA duplex r(GCAGACUUAAAUCUGC)2 with wobble C.A+ mismatches., Pan B, Mitra SN, Sundaralingam M, J Mol Biol. 1998 Nov 13;283(5):977-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9799637 9799637]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Mitra, S N.]] | | [[Category: Large Structures]] |
| [[Category: Pan, M Sundaralingam B.]] | | [[Category: Mitra SN]] |
| [[Category: a-rna double helix]] | | [[Category: Pan B]] |
| | | [[Category: Sundaralingam M]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:55 2008''
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