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| [[Image:409d.gif|left|200px]]<br /><applet load="409d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="409d, resolution 2.50Å" />
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| '''CRYSTAL STRUCTURE OF AN RNA R(CCCIUGGG) WITH THREE INDEPENDENT DUPLEXES INCORPORATING TANDEM I.U WOBBLES'''<br />
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| ==Overview== | | ==CRYSTAL STRUCTURE OF AN RNA R(CCCIUGGG) WITH THREE INDEPENDENT DUPLEXES INCORPORATING TANDEM I.U WOBBLES== |
| The crystal structure of the RNA octamer duplex r(CCCIUGGG)2has been elucidated at 2.5 A resolution. The crystals belong to the space group P21and have unit cell constants a = 33.44 A, b = 43.41 A, c = 49.39 A and beta = 104.7 degrees with three independent duplexes (duplexes 1-3) in the asymmetric unit. The structure was solved by the molecular replacement method and refined to an Rwork/Rfree of 0.185/0.243 using 3765 reflections between 8.0 and 2.5 A. This is the first report of an RNA crystal structure incorporating I.U wobbles and three molecules in the asymmetric unit. Duplex 1 displays a kink of 24 degrees between the mismatch sites, while duplexes 2 and 3 have two kinks each of 19 degrees and 27 degrees, and 24 degrees and 29 degrees, respectively, on either side of the tandem mismatches. At the I.U/U.I mismatch steps, duplex 1 has a twist angle of 33.9 degrees, close to the average for all base pair steps, but duplexes 2 and 3 are underwound, with twist angles of 24.4 degrees and 26.5 degrees, respectively. The tandem I.U wobbles show intrastrand purine-pyrimidine stacking but exhibit interstrand purine-purine stacking with the flanking C.G pairs. The three independent duplexes are stacked non-coaxially in a head-to-tail fashion to form infinite pseudo-continuous helical columns which form intercolumn hydrogen bonding interactions through the 2'-hydroxyl groups where the minor grooves come together.
| | <StructureSection load='409d' size='340' side='right'caption='[[409d]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[409d]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=409D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=409D FirstGlance]. <br> |
| 409D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=409D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=409d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=409d OCA], [https://pdbe.org/409d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=409d RCSB], [https://www.ebi.ac.uk/pdbsum/409d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=409d ProSAT]</span></td></tr> |
| ==Reference==
| | </table> |
| Crystal structure of an RNA octamer duplex r(CCCIUGGG)2 incorporating tandem I.U wobbles., Pan B, Mitra SN, Sun L, Hart D, Sundaralingam M, Nucleic Acids Res. 1998 Dec 15;26(24):5699-706. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9838002 9838002]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Hart, D.]] | | [[Category: Large Structures]] |
| [[Category: Mitra, S N.]] | | [[Category: Hart D]] |
| [[Category: Pan, B.]] | | [[Category: Mitra SN]] |
| [[Category: Sun, L.]] | | [[Category: Pan B]] |
| [[Category: Sundaralingam, M.]] | | [[Category: Sun L]] |
| [[Category: double helix]]
| | [[Category: Sundaralingam M]] |
| [[Category: i u wobbles]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:11:58 2008''
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