417d: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(9 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:417d.gif|left|200px]]<br /><applet load="417d" size="350" color="white" frame="true" align="right" spinBox="true"
caption="417d, resolution 1.500&Aring;" />
'''A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE'''<br />


==Overview==
==A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE==
The DNA hexamer d(CACGPG), in which dP is the ambivalent pyrimidine nucleoside analogue 2'-deoxy-beta-d-ribofuranosyl-(6H,8H-3, 4-dihydropyrimido[4,5-c][1,2]oxazin-7-one), crystallises as a left-handed Z-DNA duplex. X-ray analysis at 1.5 A shows that both P. A base-pairs are of the wobble type. This result appears inconsistent with other evidence from hybridisation and NMR studies of P-containing oligonucleotides, which suggests that, while P can form stable base-pairs with either A or G, thymine-like properties are more pronounced. Thermal denaturation experiments over a range of solution pH values indicate that protonation of the P.A base-pairs is unlikely to be responsible for the anomalous behaviour. No specific crystal packing effects can be identfied as an explanation, and it is concluded that base stacking and other interactions between nucleotide residues in Z-DNA are responsible.
<StructureSection load='417d' size='340' side='right'caption='[[417d]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[417d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=417D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=417D FirstGlance]. <br>
417D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=417D OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C46:6H,8H-3,4-DIHYDROPYRIMIDO[4,5-C][1,2]OXAZIN-7-0NE(CYTIDINE)-5-MONOPHOSPHATE'>C46</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=417d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=417d OCA], [https://pdbe.org/417d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=417d RCSB], [https://www.ebi.ac.uk/pdbsum/417d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=417d ProSAT]</span></td></tr>
A thymine-like base analogue forms wobble pairs with adenine in a Z-DNA duplex., Schuerman GS, Van Meervelt L, Loakes D, Brown DM, Kong Thoo Lin P, Moore MH, Salisbury SA, J Mol Biol. 1998 Oct 9;282(5):1005-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9753550 9753550]
</table>
[[Category: Protein complex]]
__TOC__
[[Category: Brown, D M.]]
</StructureSection>
[[Category: Lin, P K.T.]]
[[Category: Large Structures]]
[[Category: Loakes, D.]]
[[Category: Brown DM]]
[[Category: Meervelt, L Van.]]
[[Category: Lin PKT]]
[[Category: Moore, G S.]]
[[Category: Loakes D]]
[[Category: Moore, M H.]]
[[Category: Moore GS]]
[[Category: Schuerman, M H.]]
[[Category: Moore MH]]
[[Category: p a base-pairs]]
[[Category: Schuerman MH]]
[[Category: pyrimidine analogue]]
[[Category: Van Meervelt L]]
[[Category: z-dna]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:04 2008''

Latest revision as of 10:36, 1 March 2024

A THYMINE-LIKE BASE ANALOGUE FORMS WOBBLE PAIRS WITH ADENINE

417d, resolution 1.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=417d&oldid=4072778"