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| [[Image:422d.gif|left|200px]]<br /><applet load="422d" size="350" color="white" frame="true" align="right" spinBox="true"
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| caption="422d, resolution 2.60Å" />
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| '''5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3''''<br />
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| ==Overview== | | ==5'-R(*GP*AP*UP*CP*AP*CP*UP*UP*CP*GP*GP*U)-3'== |
| The crystal structure of an RNA dodecamer, r(GAUCACUUCGGU), was solved at 2.6 A resolution by the molecular-replacement method and refined to an R(work) of 18.8% (R(free) = 22.8%) using 2494 reflections. The dodecamer crystallized in the monoclinic space group C2, with unit-cell parameters a = 71.34, b = 39.98, c = 32.47 A, beta = 104.7 degrees and two independent strands in the asymmetric unit. The dodecamer adopts an octamer duplex structure with four 5'-overhang residues (G1A2U3C4), which form Watson-Crick base pairs with another four 5'-overhang residues of a symmetry-related duplex. The octamer duplex (ACUUCGGU) contains at its center four mismatched base pairs flanked by two Watson-Crick base pairs. The mismatched bases form two G.U wobble base pairs at the ends and two U.C base pairs at the center, with one base-base hydrogen bond N4(C).O4(U) and a water bridge connecting the N(3) of the cytosine and uridine. The present study reinforces the concept of the stability of the conformation of UUCG in RNA double-helical structures.
| | <StructureSection load='422d' size='340' side='right'caption='[[422d]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | | == Structural highlights == |
| ==About this Structure== | | <table><tr><td colspan='2'>[[422d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=422D FirstGlance]. <br> |
| 422D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=422D OCA].
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=422d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=422d OCA], [https://pdbe.org/422d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=422d RCSB], [https://www.ebi.ac.uk/pdbsum/422d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=422d ProSAT]</span></td></tr> |
| ==Reference==
| | </table> |
| Structure of the dodecamer r(GAUCACUUCGGU) with four 5'-overhang nucleotides., Eswaramoorthy S, Rao ST, Pan B, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):8-12. Epub 2003 Dec, 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14684886 14684886]
| | __TOC__ |
| [[Category: Protein complex]] | | </StructureSection> |
| [[Category: Eswaramoorthy, S.]] | | [[Category: Large Structures]] |
| [[Category: Rao, S T.]] | | [[Category: Eswaramoorthy S]] |
| [[Category: Sundaralingam, M.]] | | [[Category: Rao ST]] |
| [[Category: double helix]]
| | [[Category: Sundaralingam M]] |
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:12:03 2008''
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