3wq8: Difference between revisions
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==Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form== | |||
<StructureSection load='3wq8' size='340' side='right'caption='[[3wq8]], [[Resolution|resolution]] 2.81Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3wq8]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQ8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.81Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wq8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq8 OCA], [https://pdbe.org/3wq8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wq8 RCSB], [https://www.ebi.ac.uk/pdbsum/3wq8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wq8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q51723_9EURY Q51723_9EURY] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
One of the beta-glucosidases from Pyrococcus furiosus (BGLPf) is found to be a hyperthermophilic tetrameric enzyme that can degrade cellooligosaccharides. Recently, the crystal structures of the tetrameric and dimeric forms were solved. Here, a new monomeric form of BGLPf was constructed by removing the C-terminal region of the enzyme and its crystal structure was solved at a resolution of 2.8 A in space group P1. It was discovered that the mutant enzyme forms a unique dodecameric structure consisting of two hexameric rings in the asymmetric unit of the crystal. Under biological conditions, the mutant enzyme forms a monomer. This result helps explain how BGLPf has attained its oligomeric structure and thermostability. | |||
Monomer structure of a hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form.,Nakabayashi M, Kataoka M, Watanabe M, Ishikawa K Acta Crystallogr F Struct Biol Commun. 2014 Jul;70(Pt 7):854-9. doi:, 10.1107/S2053230X14010188. Epub 2014 Jun 18. PMID:25005077<ref>PMID:25005077</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 3wq8" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Beta-glucosidase 3D structures|Beta-glucosidase 3D structures]] | |||
*[[Galactosidase 3D structures|Galactosidase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pyrococcus furiosus]] | |||
[[Category: Ishikawa K]] | |||
[[Category: Kataoka M]] | |||
[[Category: Nakabayashi M]] | |||
[[Category: Watanabe M]] | |||
Latest revision as of 13:22, 8 November 2023
Monomer structure of hyperthermophilic beta-glucosidase mutant forming a dodecameric structure in the crystal form
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