4csf: Difference between revisions
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New page: '''Unreleased structure''' The entry 4csf is ON HOLD Authors: Olal, D., Daumke, O. Description: Structural insights into Toscana virus RNA encapsidation |
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The | ==Structural insights into Toscana virus RNA encapsidation== | ||
<StructureSection load='4csf' size='340' side='right'caption='[[4csf]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4csf]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Toscana_virus Toscana virus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CSF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CSF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.598Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4csf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4csf OCA], [https://pdbe.org/4csf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4csf RCSB], [https://www.ebi.ac.uk/pdbsum/4csf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4csf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NCAP_TOSV NCAP_TOSV] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses. | |||
Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein.,Olal D, Dick A, Woods VL Jr, Liu T, Li S, Devignot S, Weber F, Saphire EO, Daumke O Nucleic Acids Res. 2014 Mar 31. PMID:24688060<ref>PMID:24688060</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4csf" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Toscana virus]] | |||
[[Category: Daumke O]] | |||
[[Category: Olal D]] | |||
Latest revision as of 11:14, 9 May 2024
Structural insights into Toscana virus RNA encapsidation
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