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[[Image:5bna.jpg|left|200px]]<br /><applet load="5bna" size="350" color="white" frame="true" align="right" spinBox="true"
caption="5bna, resolution 2.600&Aring;" />
'''THE PRIMARY MODE OF BINDING OF CISPLATIN TO A B-DNA DODECAMER: C-G-C-G-A-A-T-T-C-G-C-G'''<br />


==Overview==
==THE PRIMARY MODE OF BINDING OF CISPLATIN TO A B-DNA DODECAMER: C-G-C-G-A-A-T-T-C-G-C-G==
<StructureSection load='5bna' size='340' side='right'caption='[[5bna]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5bna]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BNA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BNA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTN:PLATINUM+TRIAMINE+ION'>PTN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bna FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bna OCA], [https://pdbe.org/5bna PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bna RCSB], [https://www.ebi.ac.uk/pdbsum/5bna PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bna ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into pre-grown crystals of the B-DNA double-helical dodecamer C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of guanines, with what probably is an aquo bridge between Pt and the adjacent O6 atom of the same guanine. The entire guanine ring moves slightly toward the platinum site, into the major groove. Only three of the eight potential cisplatin binding sites on guanines actually are occupied, and this differential reactivity can be explained in terms of the relative freedom of motion of guanines toward the major groove. This shift of guanines upon ligation may weaken the glycosyl bond and assist in the depurination that leads to mismatch SOS repair and G.C. to T.A. transversion.
When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into pre-grown crystals of the B-DNA double-helical dodecamer C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of guanines, with what probably is an aquo bridge between Pt and the adjacent O6 atom of the same guanine. The entire guanine ring moves slightly toward the platinum site, into the major groove. Only three of the eight potential cisplatin binding sites on guanines actually are occupied, and this differential reactivity can be explained in terms of the relative freedom of motion of guanines toward the major groove. This shift of guanines upon ligation may weaken the glycosyl bond and assist in the depurination that leads to mismatch SOS repair and G.C. to T.A. transversion.


==About this Structure==
The primary mode of binding of cisplatin to a B-DNA dodecamer: C-G-C-G-A-A-T-T-C-G-C-G.,Wing RM, Pjura P, Drew HR, Dickerson RE EMBO J. 1984 May;3(5):1201-6. PMID:6539674<ref>PMID:6539674</ref>
5BNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=PTN:'>PTN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BNA OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
The primary mode of binding of cisplatin to a B-DNA dodecamer: C-G-C-G-A-A-T-T-C-G-C-G., Wing RM, Pjura P, Drew HR, Dickerson RE, EMBO J. 1984 May;3(5):1201-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=6539674 6539674]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 5bna" style="background-color:#fffaf0;"></div>
[[Category: Dickerson, R E.]]
== References ==
[[Category: Drew, H R.]]
<references/>
[[Category: Pjura, P.]]
__TOC__
[[Category: Wing, R M.]]
</StructureSection>
[[Category: PTN]]
[[Category: Large Structures]]
[[Category: b-dna]]
[[Category: Dickerson RE]]
[[Category: complexed with drug]]
[[Category: Drew HR]]
[[Category: double helix]]
[[Category: Pjura P]]
[[Category: modified]]
[[Category: Wing RM]]
 
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