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{{STRUCTURE_4mb3|  PDB=4mb3  |  SCENE=  }}
===Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella marina===
{{ABSTRACT_PUBMED_24598737}}


==About this Structure==
==Crystal structure of E153Q mutant of cold-adapted chitinase from Moritella marina==
[[4mb3]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB3 OCA].  
<StructureSection load='4mb3' size='340' side='right'caption='[[4mb3]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mb3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moritella_marina Moritella marina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MB3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MB3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mb3 OCA], [https://pdbe.org/4mb3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mb3 RCSB], [https://www.ebi.ac.uk/pdbsum/4mb3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mb3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B1VBB0_MORMI B1VBB0_MORMI]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The four-domain structure of chitinase 60 from Moritella marina (MmChi60) is outstanding in its complexity. Many glycoside hydrolases, such as chitinases and cellulases, have multi-domain structures, but only a few have been solved. The flexibility of the hinge regions between the domains apparently makes these proteins difficult to crystallize. The analysis of an active-site mutant of MmChi60 in an unliganded form and in complex with the substrates NAG4 and NAG5 revealed significant differences in the substrate-binding site compared with the previously determined complexes of most studied chitinases. A SAXS experiment demonstrated that in addition to the elongated state found in the crystal, the protein can adapt other conformations in solution ranging from fully extended to compact.


==Reference==
Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution.,Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W Acta Crystallogr D Biol Crystallogr. 2014 Mar;70(Pt 3):676-84. doi:, 10.1107/S1399004713032264. Epub 2014 Feb 15. PMID:24598737<ref>PMID:24598737</ref>
<ref group="xtra">PMID:024598737</ref><references group="xtra"/><references/>
 
[[Category: Chitinase]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Malecki, P H.]]
</div>
[[Category: Rypniewski, W.]]
<div class="pdbe-citations 4mb3" style="background-color:#fffaf0;"></div>
[[Category: Vorgias, C E.]]
 
[[Category: Alpha/beta-barrel ig-like]]
==See Also==
[[Category: Chbd]]
*[[Chitinase 3D structures|Chitinase 3D structures]]
[[Category: Chitin binding domain]]
== References ==
[[Category: Chitinase]]
<references/>
[[Category: Hydrolase]]
__TOC__
[[Category: Hydrolaze]]
</StructureSection>
[[Category: Immunoglobulin like domain]]
[[Category: Large Structures]]
[[Category: Low activity mutant]]
[[Category: Moritella marina]]
[[Category: Nag4]]
[[Category: Malecki PH]]
[[Category: Tim-barrel]]
[[Category: Rypniewski W]]
[[Category: Vorgias CE]]
Retrieved from "https://proteopedia.org/4mb3"