8drh: Difference between revisions
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== | ==HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE== | ||
<StructureSection load='8drh' size='340' side='right'caption='[[8drh]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[8drh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DRH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8drh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8drh OCA], [https://pdbe.org/8drh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8drh RCSB], [https://www.ebi.ac.uk/pdbsum/8drh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8drh ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H. | In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H. | ||
Structure of a stereoregular phosphorothioate DNA/RNA duplex.,Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216<ref>PMID:9546216</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 8drh" style="background-color:#fffaf0;"></div> | ||
[[Category: Bachelin | == References == | ||
[[Category: Dervan | <references/> | ||
[[Category: Hacia | __TOC__ | ||
[[Category: Hessler | </StructureSection> | ||
[[Category: Kessler | [[Category: Large Structures]] | ||
[[Category: Kurz | [[Category: Bachelin M]] | ||
[[Category: Dervan PB]] | |||
[[Category: Hacia JG]] | |||
[[Category: Hessler G]] | |||
[[Category: Kessler H]] | |||
[[Category: Kurz G]] | |||
Latest revision as of 10:45, 22 May 2024
HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE
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