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[[Image:8psh.gif|left|200px]]<br /><applet load="8psh" size="350" color="white" frame="true" align="right" spinBox="true"
caption="8psh" />
'''HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE'''<br />


==Overview==
==HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE==
<StructureSection load='8psh' size='340' side='right'caption='[[8psh]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[8psh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PSH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 1 model</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AS:2-DEOXY-ADENOSINE+-5-THIO-MONOPHOSPHATE'>AS</scene>, <scene name='pdbligand=GS:GUANOSINE-5-THIO-MONOPHOSPHATE'>GS</scene>, <scene name='pdbligand=PST:THYMIDINE-5-THIOPHOSPHATE'>PST</scene>, <scene name='pdbligand=SC:2-DEOXY-CYTIDINE-5-THIOPHOSPHORATE'>SC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8psh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8psh OCA], [https://pdbe.org/8psh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8psh RCSB], [https://www.ebi.ac.uk/pdbsum/8psh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8psh ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.


==About this Structure==
Structure of a stereoregular phosphorothioate DNA/RNA duplex.,Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216<ref>PMID:9546216</ref>
8PSH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=G:'>G</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PSH OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9546216 9546216]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 8psh" style="background-color:#fffaf0;"></div>
[[Category: Bachelin, M.]]
== References ==
[[Category: Dervan, P B.]]
<references/>
[[Category: Hacia, J G.]]
__TOC__
[[Category: Hessler, G.]]
</StructureSection>
[[Category: Kessler, H.]]
[[Category: Large Structures]]
[[Category: Kurz, G.]]
[[Category: Bachelin M]]
[[Category: G]]
[[Category: Dervan PB]]
[[Category: antisense]]
[[Category: Hacia JG]]
[[Category: dna/rna hybrid]]
[[Category: Hessler G]]
[[Category: oligonucleotide]]
[[Category: Kessler H]]
[[Category: phosphorothioate]]
[[Category: Kurz G]]
[[Category: thionucleotide]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 19:18:16 2008''

Latest revision as of 17:35, 25 September 2026

HIGH RESOLUTION NMR STRUCTURE OF THE STEREOREGULAR (ALL-RP)-PHOSPHOROTHIOATE-DNA/RNA HYBRID D (G*PS*C*PS*G*PS*T*PS*C*PS*A*PS*G*PS*G)R(CCUGACGC), MINIMIZED AVERAGE STRUCTURE

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