4m6f: Difference between revisions

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{{STRUCTURE_4m6f|  PDB=4m6f  |  SCENE=  }}
===Dimer of the G-Segment Invertase bound to a DNA substrate===
{{ABSTRACT_PUBMED_24598738}}


==Function==
==Dimer of the G-Segment Invertase bound to a DNA substrate==
[[http://www.uniprot.org/uniprot/DNIV_BPMU DNIV_BPMU]] This protein catalyzes the inversion of a 3000-bp segment of phage DNA. The inversion results in a modification of the 3'-end of the tail fiber gene and alters the host specificity.  
<StructureSection load='4m6f' size='340' side='right'caption='[[4m6f]], [[Resolution|resolution]] 4.99&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4m6f]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Mu Escherichia virus Mu] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4M6F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.99&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4m6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4m6f OCA], [https://pdbe.org/4m6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4m6f RCSB], [https://www.ebi.ac.uk/pdbsum/4m6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4m6f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GIN_BPMU GIN_BPMU] Performs inversion of a viral 3 kp segment (G-segment) that encodes two alternate pairs of tail fiber proteins thereby modifying the host specificity of the virus. Binds as a dimer to the viral gix sites which are 34-bp palindromic sequences that flank the invertible G-segment. Catalyzes site-specific recombination in the presence of the host factor Fis. Gin dimers bound to each of the gix sites and host factor Fis bound to the enhancer come together to form the synaptic complex. Each Gin monomer introduces a nick and becomes covalently attached to the 5'-phosphate of the DNA, resulting in double-stranded staggered breaks at both recombination sites. A 180 degrees rotation of one of the two Gin dimers followed by religation of the DNA leads to the inversion of the G-segment (G+ or G- orientation).<ref>PMID:23275567</ref> <ref>PMID:2974801</ref> <ref>PMID:3159478</ref> <ref>PMID:6232613</ref>


==About this Structure==
==See Also==
[[4m6f]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_mu Enterobacteria phage mu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4M6F OCA].
*[[Invertase|Invertase]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:024598738</ref><references group="xtra"/><references/>
__TOC__
[[Category: Enterobacteria phage mu]]
</StructureSection>
[[Category: Ritacco, C J.]]
[[Category: Escherichia virus Mu]]
[[Category: Steitz, T A.]]
[[Category: Large Structures]]
[[Category: Wang, J.]]
[[Category: Synthetic construct]]
[[Category: Catalytic domain]]
[[Category: Ritacco CJ]]
[[Category: Dna binding domain]]
[[Category: Steitz TA]]
[[Category: Helix-turn-helix]]
[[Category: Wang J]]
[[Category: Hydrolase-dna complex]]
[[Category: Recombinase]]
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