4pz1: Difference between revisions
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New page: '''Unreleased structure''' The entry 4pz1 is ON HOLD Authors: Wisniewska, M., Happonen, L., Frick, I.-M., Bjorck, L., Streicher, W., Malmstrom, J., Wikstrom, M. Description: Crystal st... |
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The | ==Crystal structure of a sHIP (UniProt Id: Q99XU0) mutant from Streptococcus pyogenes== | ||
<StructureSection load='4pz1' size='340' side='right'caption='[[4pz1]], [[Resolution|resolution]] 1.73Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4pz1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes_serotype_M1 Streptococcus pyogenes serotype M1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PZ1 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pz1 OCA], [https://pdbe.org/4pz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pz1 RCSB], [https://www.ebi.ac.uk/pdbsum/4pz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pz1 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q99XU0_STRP1 Q99XU0_STRP1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Streptococcus pyogenes is a significant bacterial pathogen in the human population. The importance of virulence factors for the survival and colonization of S. pyogenes is well established, and many of these factors are exposed to the extracellular environment enabling bacterial interactions with the host. In the present study we quantitatively analyzed and compared S. pyogenes proteins in the growth medium of a strain that is virulent to mice, with a non-virulent strain. Particularly one of these proteins was present at significantly higher levels in stationary growth medium from the virulent strain. We determined the three-dimensional structure of the protein that showed a unique tetrameric organization composed of four helix-loop-helix motifs. Affinity pull-down mass spectrometry analysis in human plasma demonstrated that the protein interacts with histidine-rich glycoprotein (HRG), and the name sHIP (streptococcal Histidine-rich glycoprotein Interacting Protein) is therefore proposed. HRG has antibacterial activity, and when challenged by HRG, sHIP was found to rescue S. pyogenes bacteria. This and the finding that patients with invasive S. pyogenes infection respond with antibody production against sHIP, suggest a role for the protein in S. pyogenes pathogenesis. | |||
Functional and Structural Properties of a Novel Protein and Virulence Factor (sHIP) in Streptococcus pyogenes.,Wisniewska M, Happonen L, Kahn F, Varjosalo M, Malmstrom L, Rosenberger G, Karlsson C, Cazzamali G, Pozdnyakova I, Frick IM, Bjorck L, Streicher W, Malmstrom J, Wikstrom M J Biol Chem. 2014 May 13. pii: jbc.M114.565978. PMID:24825900<ref>PMID:24825900</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4pz1" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptococcus pyogenes serotype M1]] | |||
[[Category: Bjorck L]] | |||
[[Category: Frick I-M]] | |||
[[Category: Happonen L]] | |||
[[Category: Malmstrom J]] | |||
[[Category: Streicher W]] | |||
[[Category: Wikstrom M]] | |||
[[Category: Wisniewska M]] | |||
Latest revision as of 17:24, 20 September 2023
Crystal structure of a sHIP (UniProt Id: Q99XU0) mutant from Streptococcus pyogenes
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