Pre-mRNA-splicing factor: Difference between revisions

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{{STRUCTURE_3e9l|  PDB=3e9l  | SIZE=400| SCENE= |right|CAPTION=Human pre-mRNA-splicing factor 8 endonuclease domain complex with Na+ (purple) and Cl- (green) ions, [[3e9l]] }}
<StructureSection load='' size='350' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'>
__TOC__
== Function ==
'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>. 


'''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing.   For details on pre-mRNA-splicing factors see<br />
*'''Prp3b''' is part of the U2 small nuclear ribonucleoproteins complex (U2 snRNP)<br />
*[[Prp8]]<br />
*'''Prp8''' functions as a scaffold which mediates the ordered assembly of spliceosomal proteins and snRNAs. For details see [[Prp8]]<br />
*[[Prp24]]<br />
*'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br />
*[[Prp19]]<br />
*'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs.  For details see [[Prp24]]<br />
*[[Prp40]].
*For '''Prp19''' see [[Prp19]]<br />
*For '''Prp20''' see [[Srp20-Human Alternative Splicing Factor]]<br />
*'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br />
*For '''Prp40''' see [[Prp40]].<br />
*'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center  '''Prp RBM22''' is the human homolog of Prpcwc2.<br />
*'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br />


{{TOC limit|limit=2}}
== Disease ==
Mutations in Prp8 and Prp31 cause retinitis pigmentosa<ref>PMID:11468273</ref>.
 
== Structural highlights ==
<scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>.


== 3D Structures of pre-mRNA splicing factors ==
== 3D Structures of pre-mRNA splicing factors ==
[[Pre-mRNA splicing factors 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
 
===Pre-mRNA-splicing factor 2===
 
[[3beg]] – hPrp residues 105-219 + Ser/Thr-protein kinase – human<BR />
[[2o3d]] – hPrp P33 subunit
 
===Pre-mRNA-splicing factor 3===
 
[[1x4q]] – hPrp PWI domain - NMR<BR />
 
===Pre-mRNA-splicing factor 3B===
 
[[3lqv]], [[2f9d]], [[2f9j]] – hPrp residues 377-415 + pre-mRNA branch site protein 14<BR />
[[2fho]] - hPrp residues 379-424 + spliceosomal protein P14 - NMR<BR />
[[2do5]] – hPrp subunit 2 – NMR<BR />
[[1x5t]] - hPrp subunit 4 – NMR<BR />
[[1x5u]] - hPrp subunit 4 RNA-binding domain – NMR<BR />
 
===Pre-mRNA-splicing factor 8===
 
[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 – yeast<BR />
[[4jk7]], [[4jk9]] - yPrp RNAseH like domain <br />
[[4ilj]], [[4jk8]], [[4jka]], [[4jkb]], [[4jkc]], [[4jkd]], [[4jke]], [[4jkf]], [[4jkg]], [[4jkh]]  - yPrp RNAseH like domain (mutant)<br />
[[3e66]] – yPrp β-finger domain<BR />
[[3e9o]], [[3e9p]] - yPrp RNA-binding domain<BR />
[[2p87]], [[2og4]], [[3sbg]] - yPrp C terminal<BR />
[[3zef]], [[4i43]] – yPrp + A1 cistron-splicing factor AAR2<br />
[[4bgd]] – yPrp JAB1/MPN domain + pre-mRNA-splicing helicase BRR2<br />
[[4kit]] – yPrp JAB1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />
[[4ilg]] – yPrp JAB1/MPN+RNAseH domains + A1 cistron-splicing factor AAR2 <br />
[[4bgd]] – yPrp JAB1/MPN+RNAseH domains + A1 cistron-splicing factor AAR2 + pre-mRNA-splicing helicase BRR2<br />
[[3lru]] – hPrp residues 1831-1990<BR />
[[3e9l]] – hPrp endonuclease domain<BR />
[[3enb]] - hPrp core domain IV<BR />
[[2p8r]] – Prp C terminal (mutant) – ''Caenorhabditis elegans''
 
===Pre-mRNA-splicing factor 9===
 
[[4dgw]] – yPrp + Prp 21 + Prp 11
 
===Pre-mRNA-splicing factor 11===
 
[[4dgw]] – yPrp + Prp 21 + Prp 9
 
===Pre-mRNA-splicing factor 18===
 
[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />
 
===Pre-mRNA-splicing factor 19===
 
[[3lrv]] – yPrp WD40 domain<BR />
[[2bay]] – yPrp U-box<BR />
[[1n87]] - yPrp U-box - NMR<BR />
 
===Pre-mRNA-splicing factor 20===
 
[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />
[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />
 
===Pre-mRNA-splicing factor 21===
 
[[4dgw]] – yPrp + Prp 9 + Prp 11
 
===snRNA-associated-splicing factor 24===
 
[[2l9w]] – yPrp C terminal – NMR<BR />
[[2kh9]] – yPrp residues 115-197 + RNA – NMR<BR />
[[2go9]] – yPrp RNA-binding  domains – NMR<BR />
[[2ghp]] - yPrp RNA-binding  domains
 
===Pre-mRNA-splicing factor 31===
 
[[2ozb]] – hPrp + RNA + tri-snRNP <br />
[[3siu]], [[3siv]] – hPrp + NHP2-like protein + snRNP <br />
 
===Pre-mRNA-splicing factor 40===
 
[[1o6w]] – yPrp WW domain – yeast - NMR<br />
[[2b7e]] – yPrp FF1 domain - NMR<br />
[[2kfd]] – yPrp FF4 domain - NMR<br />
 
===Pre-mRNA-splicing factor 43P===
 
[[2xau]], [[3kx2]] – yPrp + ADP
 
===Pre-mRNA-splicing factor cwc2===
 
[[3tp2]], [[3u1l]], [[3u1m]] – yPrp <br />
 
===Pre-mRNA-splicing factor RBM22===
 
[[2ytc]] – yPrp RNA-binding domain - NMR<BR />
 
 
===Pre-mRNA-splicing factor RDS3===
 
[[2k0a]] – yPrp - NMR<BR />
 
===Pre-mRNA-splicing factor URN1===
 
[[2juc]] – yPrp FF domain - NMR<BR />
 
 


== References ==
<references/>




[[Category:Topic Page]]
[[Category:Topic Page]]
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