4mqx: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
{{STRUCTURE_4mqx|  PDB=4mqx  |  SCENE=  }}
===CLC-ec1 Fab Complex Cysless A399C-A432C mutant===


==Function==
==CLC-ec1 Fab Complex Cysless A399C-A432C mutant==
[[http://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
<StructureSection load='4mqx' size='340' side='right'caption='[[4mqx]], [[Resolution|resolution]] 3.52&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4mqx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MQX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.516&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mqx OCA], [https://pdbe.org/4mqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mqx RCSB], [https://www.ebi.ac.uk/pdbsum/4mqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mqx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
CLC-type exchangers mediate transmembrane Cl(-) transport. Mutations altering their gating properties cause numerous genetic disorders. However, their transport mechanism remains poorly understood. In conventional models, two gates alternatively expose substrates to the intra- or extracellular solutions. A glutamate was identified as the only gate in the CLCs, suggesting that CLCs function by a nonconventional mechanism. Here we show that transport in CLC-ec1, a prokaryotic homolog, is inhibited by cross-links constraining movement of helix O far from the transport pathway. Cross-linked CLC-ec1 adopts a wild-type-like structure, indicating stabilization of a native conformation. Movements of helix O are transduced to the ion pathway via a direct contact between its C terminus and a tyrosine that is a constitutive element of the second gate of CLC transporters. Therefore, the CLC exchangers have two gates that are coupled through conformational rearrangements outside the ion pathway.


==About this Structure==
Conformational changes required for H(+)/Cl(-) exchange mediated by a CLC transporter.,Basilio D, Noack K, Picollo A, Accardi A Nat Struct Mol Biol. 2014 May;21(5):456-63. doi: 10.1038/nsmb.2814. Epub 2014 Apr, 20. PMID:24747941<ref>PMID:24747941</ref>
[[4mqx]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MQX OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
<references group="xtra"/><references/>
</div>
[[Category: Ecoli]]
<div class="pdbe-citations 4mqx" style="background-color:#fffaf0;"></div>
[[Category: Lk3 transgenic mice]]
== References ==
[[Category: Accardi, A.]]
<references/>
[[Category: Basilio, D.]]
__TOC__
[[Category: Noack, K.]]
</StructureSection>
[[Category: Picollo, A.]]
[[Category: Escherichia coli K-12]]
[[Category: Alpha helical transmembrane protein]]
[[Category: Large Structures]]
[[Category: Cl/h antiporter]]
[[Category: Mus musculus]]
[[Category: Membrane protein]]
[[Category: Accardi A]]
[[Category: Metal transport]]
[[Category: Basilio D]]
[[Category: Noack K]]
[[Category: Picollo A]]
Retrieved from "https://proteopedia.org/4mqx"