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New page: left|200px<br /><applet load="3btu" size="350" color="white" frame="true" align="right" spinBox="true" caption="3btu, resolution 2.85Å" /> '''Crystal structure of...
 
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[[Image:3btu.jpg|left|200px]]<br /><applet load="3btu" size="350" color="white" frame="true" align="right" spinBox="true"
caption="3btu, resolution 2.85&Aring;" />
'''Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S2) [E351K]'''<br />


==Overview==
==Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S2) [E351K]==
Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.
<StructureSection load='3btu' size='340' side='right'caption='[[3btu]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3btu]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BTU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3btu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3btu OCA], [https://pdbe.org/3btu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3btu RCSB], [https://www.ebi.ac.uk/pdbsum/3btu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3btu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GAL80_YEAST GAL80_YEAST] This protein is a negative regulator for the gene expression of the lactose/galactose metabolic genes. It binds to GAL4 and so blocks transcriptional activation by it, in the absence of an inducing sugar.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bt/3btu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3btu ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
3BTU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BTU OCA].
*[[Gal3-Gal80-Gal4|Gal3-Gal80-Gal4]]
 
__TOC__
==Reference==
</StructureSection>
NADP regulates the yeast GAL induction system., Kumar PR, Yu Y, Sternglanz R, Johnston SA, Joshua-Tor L, Science. 2008 Feb 22;319(5866):1090-2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18292341 18292341]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Joshua-Tor L]]
[[Category: Joshua-Tor, L.]]
[[Category: Kumar PR]]
[[Category: Kumar, P R.]]
[[Category: acetylation]]
[[Category: carbohydrate metabolism]]
[[Category: dna-binding]]
[[Category: eukaryotic transcription complex]]
[[Category: galactose metabolism]]
[[Category: repressor]]
[[Category: transcription regulation]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar  5 13:24:09 2008''

Latest revision as of 09:31, 21 February 2024

Crystal structure of the super-repressor mutant of Gal80p from Saccharomyces cerevisiae; Gal80(S2) [E351K]

3btu, resolution 2.85Å

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