3wtf: Difference between revisions

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~~'''Unreleased structure'''~~

~~The entry~~ 3wtf is ~~ON HOLD~~

==Structure of PAXX==

== Structural highlights ==

<table><tr><td colspan='2'>[[3wtf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WTF FirstGlance]. <br>

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.451Å</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wtf OCA], [https://pdbe.org/3wtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wtf RCSB], [https://www.ebi.ac.uk/pdbsum/3wtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wtf ProSAT]</span></td></tr>

</table>

== Function ==

[https://www.uniprot.org/uniprot/PAXX_HUMAN PAXX_HUMAN] Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ); participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:27705800). May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:25574025, PubMed:25670504, PubMed:25941166). Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (PubMed:27703001). Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed:30250067, PubMed:25670504). Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27705800). Able to restrict infection by herpesvirus 1 (HSV-1) via an unknown mechanism (PubMed:29144403).<ref>PMID:25574025</ref> <ref>PMID:25670504</ref> <ref>PMID:25941166</ref> <ref>PMID:27703001</ref> <ref>PMID:27705800</ref> <ref>PMID:29144403</ref> <ref>PMID:30250067</ref>

== Publication Abstract from PubMed ==

XRCC4 and XLF are two structurally related proteins that function in DNA double-strand break (DSB) repair. Here, we identify human PAXX (PAralog of XRCC4 and XLF, also called C9orf142) as a new XRCC4 superfamily member and show that its crystal structure resembles that of XRCC4. PAXX interacts directly with the DSB-repair protein Ku and is recruited to DNA-damage sites in cells. Using RNA interference and CRISPR-Cas9 to generate PAXX(-/-) cells, we demonstrate that PAXX functions with XRCC4 and XLF to mediate DSB repair and cell survival in response to DSB-inducing agents. Finally, we reveal that PAXX promotes Ku-dependent DNA ligation in vitro and assembly of core nonhomologous end-joining (NHEJ) factors on damaged chromatin in cells. These findings identify PAXX as a new component of the NHEJ machinery.

~~Authors:~~ Ochi, T., Blundell, T.L.

DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.,Ochi T, Blackford AN, Coates J, Jhujh S, Mehmood S, Tamura N, Travers J, Wu Q, Draviam VM, Robinson CV, Blundell TL, Jackson SP Science. 2015 Jan 9;347(6218):185-188. doi: 10.1126/science.1261971. PMID:25574025<ref>PMID:25574025</ref>

~~Description~~: ~~Structure of PAXX~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Homo sapiens]]

[[Category: Large Structures]]

[[Category: Blundell TL]]

[[Category: Ochi T]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 3wtf is ON HOLD
+==Structure of PAXX==
+<StructureSection load='3wtf' size='340' side='right'caption='[[3wtf]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3wtf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WTF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WTF FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.451&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wtf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wtf OCA], [https://pdbe.org/3wtf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wtf RCSB], [https://www.ebi.ac.uk/pdbsum/3wtf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wtf ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PAXX_HUMAN PAXX_HUMAN] Non-essential DNA repair protein involved in DNA non-homologous end joining (NHEJ); participates in double-strand break (DSB) repair and V(D)J recombination (PubMed:25574025, PubMed:25670504, PubMed:25941166, PubMed:27705800). May act as a scaffold required for accumulation of the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites and promote the assembly and/or stability of the NHEJ machinery (PubMed:25574025, PubMed:25670504, PubMed:25941166). Involved in NHEJ by promoting the ligation of blunt-ended DNA ends (PubMed:27703001). Together with NHEJ1/XLF, collaborates with DNA polymerase lambda (POLL) to promote joining of non-cohesive DNA ends (PubMed:30250067, PubMed:25670504). Constitutes a non-essential component of classical NHEJ: has a complementary but distinct function with NHEJ1/XLF in DNA repair (PubMed:27705800). Able to restrict infection by herpesvirus 1 (HSV-1) via an unknown mechanism (PubMed:29144403).<ref>PMID:25574025</ref> <ref>PMID:25670504</ref> <ref>PMID:25941166</ref> <ref>PMID:27703001</ref> <ref>PMID:27705800</ref> <ref>PMID:29144403</ref> <ref>PMID:30250067</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+XRCC4 and XLF are two structurally related proteins that function in DNA double-strand break (DSB) repair. Here, we identify human PAXX (PAralog of XRCC4 and XLF, also called C9orf142) as a new XRCC4 superfamily member and show that its crystal structure resembles that of XRCC4. PAXX interacts directly with the DSB-repair protein Ku and is recruited to DNA-damage sites in cells. Using RNA interference and CRISPR-Cas9 to generate PAXX(-/-) cells, we demonstrate that PAXX functions with XRCC4 and XLF to mediate DSB repair and cell survival in response to DSB-inducing agents. Finally, we reveal that PAXX promotes Ku-dependent DNA ligation in vitro and assembly of core nonhomologous end-joining (NHEJ) factors on damaged chromatin in cells. These findings identify PAXX as a new component of the NHEJ machinery.
-Authors: Ochi, T., Blundell, T.L.
+DNA repair. PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair.,Ochi T, Blackford AN, Coates J, Jhujh S, Mehmood S, Tamura N, Travers J, Wu Q, Draviam VM, Robinson CV, Blundell TL, Jackson SP Science. 2015 Jan 9;347(6218):185-188. doi: 10.1126/science.1261971. PMID:25574025<ref>PMID:25574025</ref>
-Description: Structure of PAXX
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 3wtf" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Blundell TL]]
+[[Category: Ochi T]]