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{{STRUCTURE_4mrt|  PDB=4mrt  |  SCENE=  }}
===Structure of the Phosphopantetheine Transferase Sfp in Complex with Coenzyme A and a Peptidyl Carrier Protein===
{{ABSTRACT_PUBMED_24704508}}


==Function==
==Structure of the Phosphopantetheine Transferase Sfp in Complex with Coenzyme A and a Peptidyl Carrier Protein==
[[http://www.uniprot.org/uniprot/TYCC_BREPA TYCC_BREPA]] Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product. [[http://www.uniprot.org/uniprot/SFP_BACSU SFP_BACSU]] Activates the seven peptidyl carrier protein (PCP) domains of surfactin synthase SRF1/2/3 by transferring the 4'-phosphopantetheinyl moiety of coenzyme A (CoA) to a serine residue. Required for cells of B.subtilis to become producers of the lipopeptide antibiotics surfactin and plipastatin B1.<ref>PMID:8939709</ref> 
<StructureSection load='4mrt' size='340' side='right'caption='[[4mrt]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[4mrt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168] and [https://en.wikipedia.org/wiki/Brevibacillus_parabrevis Brevibacillus parabrevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MRT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MRT FirstGlance]. <br>
[[4mrt]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MRT OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mrt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mrt OCA], [https://pdbe.org/4mrt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mrt RCSB], [https://www.ebi.ac.uk/pdbsum/4mrt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mrt ProSAT]</span></td></tr>
<ref group="xtra">PMID:024704508</ref><references group="xtra"/><references/>
</table>
[[Category: Dikic, I.]]
== Function ==
[[Category: Doetsch, V.]]
[https://www.uniprot.org/uniprot/TYCC_BREPA TYCC_BREPA] Incorporates six amino acids (for tyrocidine A, Asn, Gln, Tyr, Val, Orn, and Leu) in their L-configuration into the peptide product.
[[Category: Guentert, P.]]
__TOC__
[[Category: Henrich, E.]]
</StructureSection>
[[Category: Kirchner, D K.]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Koepke, J.]]
[[Category: Brevibacillus parabrevis]]
[[Category: Kraas, F I.]]
[[Category: Large Structures]]
[[Category: Loehr, F.]]
[[Category: Dikic I]]
[[Category: Marahiel, M A.]]
[[Category: Doetsch V]]
[[Category: Rahighi, S.]]
[[Category: Guentert P]]
[[Category: Tufar, P.]]
[[Category: Henrich E]]
[[Category: Pcp: aminoacyl/peptidyl carrier]]
[[Category: Kirchner DK]]
[[Category: Sfp: coenzyme a binding]]
[[Category: Koepke J]]
[[Category: Sfp: phosphopantetheine transferase]]
[[Category: Kraas FI]]
[[Category: Transport protein-transferase complex]]
[[Category: Loehr F]]
[[Category: Marahiel MA]]
[[Category: Rahighi S]]
[[Category: Tufar P]]

Latest revision as of 12:28, 1 March 2024

Structure of the Phosphopantetheine Transferase Sfp in Complex with Coenzyme A and a Peptidyl Carrier Protein

4mrt, resolution 2.00Å

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