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New page: left|200px<br /><applet load="3bwd" size="350" color="white" frame="true" align="right" spinBox="true" caption="3bwd, resolution 1.53Å" /> '''Crystal structure of...
 
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[[Image:3bwd.jpg|left|200px]]<br /><applet load="3bwd" size="350" color="white" frame="true" align="right" spinBox="true"
caption="3bwd, resolution 1.53&Aring;" />
'''Crystal structure of the plant Rho protein ROP5'''<br />


==Overview==
==Crystal structure of the plant Rho protein ROP5==
The small G protein ROP5 from the model plant Arabidopsis thaliana was purified and crystallized using the hanging-drop vapour-diffusion method. ROP5 crystals were obtained using PEG 3000 as precipitant and belong to space group P2(1). A data set was collected to 1.53 A resolution using synchrotron radiation at 100 K. A clear molecular-replacement solution was found using ROP4-GDP of the ROP4-GDP-PRONE8 complex as the search model.
<StructureSection load='3bwd' size='340' side='right'caption='[[3bwd]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3bwd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BWD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bwd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwd OCA], [https://pdbe.org/3bwd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bwd RCSB], [https://www.ebi.ac.uk/pdbsum/3bwd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bwd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RAC6_ARATH RAC6_ARATH] May be involved in cell polarity control during the actin-dependent tip growth of pollen tubes.<ref>PMID:10209027</ref> <ref>PMID:9765526</ref>  Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation (By similarity).<ref>PMID:10209027</ref> <ref>PMID:9765526</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bwd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bwd ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
3BWD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=GDP:'>GDP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+D+181'>AC1</scene> and <scene name='pdbsite=AC2:Gdp+Binding+Site+For+Residue+D+200'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWD OCA].
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
 
== References ==
==Reference==
<references/>
Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5., Thomas C, Berken A, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1070-2. Epub 2007 Nov 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18084097 18084097]
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Arabidopsis thaliana]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Berken, A.]]
[[Category: Berken A]]
[[Category: Thomas, C.]]
[[Category: Thomas C]]
[[Category: GDP]]
[[Category: MG]]
[[Category: cytoplasm]]
[[Category: g domain]]
[[Category: gtp-binding]]
[[Category: lipoprotein]]
[[Category: membrane]]
[[Category: methylation]]
[[Category: nucleotide-binding]]
[[Category: plant protein]]
[[Category: prenylation]]
 
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Retrieved from "https://proteopedia.org/3bwd"