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==HlyU from Vibrio cholerae N16961==
==HlyU from Vibrio cholerae N16961==
<StructureSection load='4k2e' size='340' side='right' caption='[[4k2e]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4k2e' size='340' side='right'caption='[[4k2e]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
[[4k2e]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K2E OCA]. <br>
<table><tr><td colspan='2'>[[4k2e]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_biovar_El_Tor_str._N16961 Vibrio cholerae O1 biovar El Tor str. N16961]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K2E FirstGlance]. <br>
<b>[[Non-Standard_Residue|NonStd Res:]]</b> <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<b>Activity:</b> <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span><br>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr>
<b>Resources:</b> <span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4k2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k2e OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4k2e RCSB], [http://www.ebi.ac.uk/pdbsum/4k2e PDBsum]</span><br>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k2e OCA], [https://pdbe.org/4k2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k2e RCSB], [https://www.ebi.ac.uk/pdbsum/4k2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k2e ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HLYU_VIBCH HLYU_VIBCH] Up-regulates the expression of the hemolysin gene, hlyA, and may promote expression of other virulence determinants in vivo. It may have both positive and negative regulator activities.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
HlyU in Vibrio cholerae is known to be the transcriptional activator of the hemolysin gene, HlyA and possibly a regulator of other virulence factors influencing growth, colonization and pathogenicity of this infective agent. Here we report the crystal structure of HlyU from V. cholerae N16961 (HlyU_Vc) at 1.8A. The protein, with five alpha-helices and three beta-strands in the topology of alpha1-alpha2-beta1-alpha3-alpha4-beta2-beta3-alpha5, forms a homodimer. Helices alpha3-alpha4 and a beta sheet form the winged helix-turn-helix (wHTH) DNA-binding motif common to the transcription regulators of the SmtB/ArsR family. In spite of an overall fold similar to SmtB/ArsR family, it lacks any metal binding site seen in SmtB. A comparison of the dimeric interfaces showed that the one in SmtB is much larger and have salt bridges that can be disrupted to accommodate metal ions. A model of HlyU-DNA complex suggests bending of the DNA. Cys38 in the structure was found to be modified as sulfenic acid; the oxidized form was not seen in another structure solved under reducing condition. Although devoid of any metal binding site, the presence of a Cys residue exhibiting oxidation-reduction suggests the possibility of the existence of a redox switch in transcription regulation. A structure-based phylogenetic analysis of wHTH proteins revealed the segregation of metal and non-metal binding proteins as well as those in the latter group that are under redox control.
 
Crystal structure of HlyU, the hemolysin gene transcription activator, from Vibrio cholerae N16961 and functional implications.,Mukherjee D, Datta AB, Chakrabarti P Biochim Biophys Acta. 2014 Oct 18;1844(12):2346-2354. doi:, 10.1016/j.bbapap.2014.09.020. PMID:25450504<ref>PMID:25450504</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4k2e" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chakrabarti, P.]]
[[Category: Large Structures]]
[[Category: Datta, A B.]]
[[Category: Vibrio cholerae O1 biovar El Tor str. N16961]]
[[Category: Mukherjee, D.]]
[[Category: Chakrabarti P]]
[[Category: Dna]]
[[Category: Datta AB]]
[[Category: Dna-binding domain]]
[[Category: Mukherjee D]]
[[Category: Hemolysin gene transcription regulator]]
[[Category: S-hydroxycysteine]]
[[Category: Transcription activator]]
[[Category: Winged helix]]
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