4nag: Difference between revisions
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==Xanthomonins I III are a New Class of Lasso Peptides Featuringa Seven-Membered Macrolactam Ring== | ==Xanthomonins I III are a New Class of Lasso Peptides Featuringa Seven-Membered Macrolactam Ring== | ||
<StructureSection load='4nag' size='340' side='right' caption='[[4nag]], [[Resolution|resolution]] 0.81Å' scene=''> | <StructureSection load='4nag' size='340' side='right'caption='[[4nag]], [[Resolution|resolution]] 0.81Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
[[4nag]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAG OCA]. <br> | <table><tr><td colspan='2'>[[4nag]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Xanthomonas_gardneri_ATCC_19865 Xanthomonas gardneri ATCC 19865]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NAG FirstGlance]. <br> | ||
<b>[[Ligand|Ligands:]]</b> <scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>< | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.81Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene></td></tr> | |||
<b>Resources:</b> <span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nag OCA], [https://pdbe.org/4nag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nag RCSB], [https://www.ebi.ac.uk/pdbsum/4nag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nag ProSAT]</span></td></tr> | ||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Lasso peptides belong to the class of ribosomally synthesized and post-translationally modified peptides. Their common distinguishing feature is an N-terminal macrolactam ring that is threaded by the C-terminal tail. This lasso fold is maintained through steric interactions. The isolation and characterization of xanthomonins I-III, the first lasso peptides featuring macrolactam rings consisting of only seven amino acids, is now presented. The crystal structure of xanthomonin I and the NMR structure of xanthomonin II were also determined. A total of 25 variants of xanthomonin II were generated to probe different aspects of the biosynthesis, stability, and fold maintenance. These mutational studies reveal the limits such a small ring imposes on the threading and show that every plug amino acid larger than serine is able to maintain a heat-stable lasso fold in the xanthomonin II scaffold. | Lasso peptides belong to the class of ribosomally synthesized and post-translationally modified peptides. Their common distinguishing feature is an N-terminal macrolactam ring that is threaded by the C-terminal tail. This lasso fold is maintained through steric interactions. The isolation and characterization of xanthomonins I-III, the first lasso peptides featuring macrolactam rings consisting of only seven amino acids, is now presented. The crystal structure of xanthomonin I and the NMR structure of xanthomonin II were also determined. A total of 25 variants of xanthomonin II were generated to probe different aspects of the biosynthesis, stability, and fold maintenance. These mutational studies reveal the limits such a small ring imposes on the threading and show that every plug amino acid larger than serine is able to maintain a heat-stable lasso fold in the xanthomonin II scaffold. | ||
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Xanthomonins I-III: A New Class of Lasso Peptides with a Seven-Residue Macrolactam Ring.,Hegemann JD, Zimmermann M, Zhu S, Steuber H, Harms K, Xie X, Marahiel MA Angew Chem Int Ed Engl. 2014 Jan 20. doi: 10.1002/anie.201309267. PMID:24446383<ref>PMID:24446383</ref> | Xanthomonins I-III: A New Class of Lasso Peptides with a Seven-Residue Macrolactam Ring.,Hegemann JD, Zimmermann M, Zhu S, Steuber H, Harms K, Xie X, Marahiel MA Angew Chem Int Ed Engl. 2014 Jan 20. doi: 10.1002/anie.201309267. PMID:24446383<ref>PMID:24446383</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
<div class="pdbe-citations 4nag" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Xanthomonas gardneri ATCC 19865]] | ||
[[Category: | [[Category: Harms K]] | ||
[[Category: | [[Category: Hegemann JD]] | ||
[[Category: | [[Category: Marahiel MA]] | ||
[[Category: | [[Category: Steuber H]] | ||
[[Category: | [[Category: Xie X]] | ||
[[Category: | [[Category: Zhu S]] | ||
[[Category: | [[Category: Zimmermann M]] | ||
