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| ==NMR model of the first let-7 miRNA complementary site (LCS1) in 3'-UTR of lin-41 mRNA from C. elegans== | | ==NMR model of the first let-7 miRNA complementary site (LCS1) in 3'-UTR of lin-41 mRNA from C. elegans== |
| <StructureSection load='2kpv' size='340' side='right' caption='[[2kpv]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | | <StructureSection load='2kpv' size='340' side='right'caption='[[2kpv]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2kpv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KPV OCA]. <br> | | <table><tr><td colspan='2'>[[2kpv]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KPV FirstGlance]. <br> |
| </td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kpv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kpv RCSB], [http://www.ebi.ac.uk/pdbsum/2kpv PDBsum]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kpv OCA], [https://pdbe.org/2kpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kpv RCSB], [https://www.ebi.ac.uk/pdbsum/2kpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kpv ProSAT]</span></td></tr> |
| <table>
| | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| We have determined the 3D structure of a 34-nt RNA construct, herein named LCS1co, which mimics the interaction of let-7 microRNA (miRNA) to one of its complementary binding sites, LCS1, in the 3'-untranslated region of lin-41 mRNA by solution-state NMR spectroscopy. let-7 miRNAs control the timing of development of the nematode Caenorhabditis elegans and are highly conserved in mammals. The sequence and structure of the two conserved let-7 complementary sites, LCS1 and LCS2, in the 3'-untranslated region of lin-41 mRNA are important for a proper downregulation of lin-41. The high-resolution NMR structure reveals details of the binding of let-7 miRNA to lin-41 mRNA which involves formation of a complex with non-canonical structural elements within the seed region. LCS1co exhibits a stem-loop structure with two stems, an asymmetric internal loop and an adenine bulge. Comparison with the NMR solution-state structure of the let-7:lin-41 complex involving the LCS2-binding site shows that conformational freedom of the asymmetric internal loop of LCS1co correlates with a smaller bend between the upper and lower stems in comparison to the well-defined asymmetric loop of LCS2co.
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| NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans.,Cevec M, Thibaudeau C, Plavec J Nucleic Acids Res. 2010 Nov;38(21):7814-21. Epub 2010 Jul 26. PMID:20660479<ref>PMID:20660479</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div> | |
| == References ==
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| <references/> | |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Cevec, M.]] | | [[Category: Large Structures]] |
| [[Category: Plavec, J.]] | | [[Category: Cevec M]] |
| [[Category: Thibaudeau, C.]] | | [[Category: Plavec J]] |
| [[Category: Adenine bulge]] | | [[Category: Thibaudeau C]] |
| [[Category: Asymmetric internal loop]]
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| [[Category: Gaaa tetraloop]]
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| [[Category: Residual dipolar coupling]]
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| [[Category: Rna]]
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| [[Category: Stem-loop]]
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