4d2i: Difference between revisions
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==Crystal structure of the HerA hexameric DNA translocase from Sulfolobus solfataricus bound to AMP-PNP== | |||
<StructureSection load='4d2i' size='340' side='right'caption='[[4d2i]], [[Resolution|resolution]] 2.84Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4d2i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D2I FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.841Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d2i OCA], [https://pdbe.org/4d2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d2i RCSB], [https://www.ebi.ac.uk/pdbsum/4d2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d2i ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HERA_SACS2 HERA_SACS2] Involved in DNA double-strand break (DSB) repair (PubMed:22135300). Acts probably with NurA to stimulate resection of the 5' strand and produce the long 3' single-strand that is required for RadA loading (By similarity). Exhibits DNA-dependent ATPase activity and DNA helicase activity (PubMed:22135300, PubMed:25420454).[UniProtKB:Q8U1P0]<ref>PMID:22135300</ref> <ref>PMID:25420454</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The HerA ATPase cooperates with the NurA nuclease and the Mre11-Rad50 complex for the repair of double-strand DNA breaks in thermophilic archaea. Here we extend our structural knowledge of this minimal end-resection apparatus by presenting the first crystal structure of hexameric HerA. The full-length structure visualizes at atomic resolution the N-terminal HerA-ATP synthase domain and a conserved C-terminal extension, which acts as a physical brace between adjacent protomers. The brace also interacts in trans with nucleotide-binding residues of the neighbouring subunit. Our observations support a model in which the coaxial interaction of the HerA ring with the toroidal NurA dimer generates a continuous channel traversing the complex. HerA-driven translocation would propel the DNA towards the narrow annulus of NurA, leading to duplex melting and nucleolytic digestion. This system differs substantially from the bacterial end-resection paradigms. Our findings suggest a novel mode of DNA-end processing by this integrated archaeal helicase-nuclease machine. | |||
Structure of the hexameric HerA ATPase reveals a mechanism of translocation-coupled DNA-end processing in archaea.,Rzechorzek NJ, Blackwood JK, Bray SM, Maman JD, Pellegrini L, Robinson NP Nat Commun. 2014 Nov 25;5:5506. doi: 10.1038/ncomms6506. PMID:25420454<ref>PMID:25420454</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4d2i" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Saccharolobus solfataricus P2]] | |||
[[Category: Blackwood JK]] | |||
[[Category: Bray SM]] | |||
[[Category: Maman JD]] | |||
[[Category: Pellegrini L]] | |||
[[Category: Robinson NP]] | |||
[[Category: Rzechorzek NJ]] | |||
Latest revision as of 11:16, 9 May 2024
Crystal structure of the HerA hexameric DNA translocase from Sulfolobus solfataricus bound to AMP-PNP
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