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==Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes==
==Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes==
<StructureSection load='3bw3' size='340' side='right' caption='[[3bw3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3bw3' size='340' side='right'caption='[[3bw3]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3bw3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_ansochromogenes Streptomyces ansochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BW3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3bw3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_ansochromogenes Streptomyces ansochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BW3 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NIE:NITROETHANE'>NIE</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3bw2|3bw2]], [[3bw4|3bw4]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NIE:NITROETHANE'>NIE</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">2-npdl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=115647 Streptomyces ansochromogenes])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw3 OCA], [https://pdbe.org/3bw3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bw3 RCSB], [https://www.ebi.ac.uk/pdbsum/3bw3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bw3 ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitroalkane_oxidase Nitroalkane oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.1 1.7.3.1] </span></td></tr>
</table>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bw3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bw3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3bw3 RCSB], [http://www.ebi.ac.uk/pdbsum/3bw3 PDBsum]</span></td></tr>
== Function ==
<table>
[https://www.uniprot.org/uniprot/Q9FDD4_9ACTN Q9FDD4_9ACTN]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bw3_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bw3_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bw3 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.,Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069<ref>PMID:21147069</ref>
Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.,Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H Biochem Biophys Res Commun. 2011 Feb 18;405(3):344-8. Epub 2010 Dec 11. PMID:21147069<ref>PMID:21147069</ref>


From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 3bw3" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Nitroalkane oxidase]]
[[Category: Large Structures]]
[[Category: Streptomyces ansochromogenes]]
[[Category: Streptomyces ansochromogenes]]
[[Category: Gao, Z Q.]]
[[Category: Gao ZQ]]
[[Category: Hou, H F.]]
[[Category: Hou HF]]
[[Category: Li, Y H.]]
[[Category: Li YH]]
[[Category: Dioxygenase]]
[[Category: Oxidoreductase]]
[[Category: Tim barrel]]

Latest revision as of 14:52, 1 November 2023

Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

3bw3, resolution 2.20Å

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