4pne: Difference between revisions

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'''Unreleased structure'''


The entry 4pne is ON HOLD
==Crystal Structure of the [4+2]-Cyclase SpnF==
<StructureSection load='4pne' size='340' side='right'caption='[[4pne]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pne]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharopolyspora_spinosa Saccharopolyspora spinosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PNE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pne OCA], [https://pdbe.org/4pne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pne RCSB], [https://www.ebi.ac.uk/pdbsum/4pne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pne ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9ALM7_SACSN Q9ALM7_SACSN]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the biosynthetic pathway of the spinosyn insecticides, the tailoring enzyme SpnF performs a [4 + 2] cycloaddition on a 22-membered macrolactone to forge an embedded cyclohexene ring. To learn more about this reaction, which could potentially proceed through a Diels-Alder mechanism, we determined the 1.50-A-resolution crystal structure of SpnF bound to S-adenosylhomocysteine. This sets the stage for advanced experimental and computational studies to determine the precise mechanism of SpnF-mediated cyclization.


Authors: Fage, C.D., Isiorho, E.A., Liu, Y.-N., Liu, H.-W., Keatinge-Clay, A.T.
The structure of SpnF, a standalone enzyme that catalyzes [4 + 2] cycloaddition.,Fage CD, Isiorho EA, Liu Y, Wagner DT, Liu HW, Keatinge-Clay AT Nat Chem Biol. 2015 Mar 2. doi: 10.1038/nchembio.1768. PMID:25730549<ref>PMID:25730549</ref>


Description: Crystal Structure of the [4+2]-Cyclase SpnF
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pne" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharopolyspora spinosa]]
[[Category: Fage CD]]
[[Category: Isiorho EA]]
[[Category: Keatinge-Clay AT]]
[[Category: Liu H-W]]
[[Category: Liu Y-N]]
Retrieved from "https://proteopedia.org/4pne"