1bn9: Difference between revisions
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==RESPONSE ELEMENT OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB BETA== | |||
<StructureSection load='1bn9' size='340' side='right'caption='[[1bn9]]' scene=''> | |||
== Structural highlights == | |||
| | <table><tr><td colspan='2'>[[1bn9]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BN9 FirstGlance]. <br> | ||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bn9 OCA], [https://pdbe.org/1bn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bn9 RCSB], [https://www.ebi.ac.uk/pdbsum/1bn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bn9 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
''' | == Publication Abstract from PubMed == | ||
== | |||
Rev-erb beta is an orphan receptor that binds as a homodimer or as a monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), the response element of Rev-erb beta for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints for distances and NOE back-calculation methods. The refined final structures have the typical overall features of B-type DNA. However, titration of this 15 bp duplex with ReDBD, the DNA binding domain of Rev-erb beta, showed large shifts of imino protons and 31P signals, suggesting major conformational changes. | Rev-erb beta is an orphan receptor that binds as a homodimer or as a monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), the response element of Rev-erb beta for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints for distances and NOE back-calculation methods. The refined final structures have the typical overall features of B-type DNA. However, titration of this 15 bp duplex with ReDBD, the DNA binding domain of Rev-erb beta, showed large shifts of imino protons and 31P signals, suggesting major conformational changes. | ||
Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation*.,Castagne C, Terenzi H, Zakin MM, Delepierre M Biochimie. 2000 Aug;82(8):739-48. PMID:11018291<ref>PMID:11018291</ref> | |||
Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation*., Castagne C, Terenzi H, Zakin MM, Delepierre M | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1bn9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Castagne C]] | |||
[[Category: Delepierre M]] | |||
[[Category: Terenzi H]] | |||
[[Category: Zakin MM]] | |||
