4q5e

Shigella Effector Kinase OspG bound to E2-Ub UbcH7-Ub Conjugate

spinqualitylabelsall models 4q5e, resolution 1.87Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4q5e is a 3 chain structure with sequence from Homo sapiens, Saccharomyces cerevisiae S288C and Shigella sonnei Ss046. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.869Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function OSPG_SHISS Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues. This protein is a kinase that is involved in down-regulation of the host innate response induced by invasive bacteria (By similarity). Publication Abstract from PubMed Shigella invasion of its human host is assisted by T3SS-delivered effector proteins. The OspG effector kinase binds ubiquitin and ubiquitin-loaded E2-conjugating enzymes, including UbcH5b and UbcH7, and attenuates the host innate immune NF-kB signaling. We present the structure of OspG bound to the UbcH7 approximately Ub conjugate. OspG has a minimal kinase fold lacking the activation loop of regulatory kinases. UbcH7 approximately Ub binds OspG at sites remote from the kinase active site, yet increases its kinase activity. The ubiquitin is positioned in the "open" conformation with respect to UbcH7 using its I44 patch to interact with the C terminus of OspG. UbcH7 binds to OspG using two conserved loops essential for E3 ligase recruitment. The interaction of the UbcH7 approximately Ub with OspG is remarkably similar to the interaction of an E2 approximately Ub with a HECT E3 ligase. OspG interferes with the interaction of UbcH7 with the E3 parkin and inhibits the activity of the E3. Structural Basis for the Inhibition of Host Protein Ubiquitination by Shigella Effector Kinase OspG.,Grishin AM, Condos TE, Barber KR, Campbell-Valois FX, Parsot C, Shaw GS, Cygler M Structure. 2014 Jun 10;22(6):878-88. doi: 10.1016/j.str.2014.04.010. Epub 2014, May 22. PMID:24856362 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 3D structures of ubiquitin 3D structures of ubiquitin conjugating enzyme

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