2mp9: Difference between revisions
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==Solution structure of an potent antifungal peptide Cm-p5 derived from C. muricatus== | |||
<StructureSection load='2mp9' size='340' side='right'caption='[[2mp9]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2mp9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cenchritis_muricatus Cenchritis muricatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MP9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MP9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mp9 OCA], [https://pdbe.org/2mp9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mp9 RCSB], [https://www.ebi.ac.uk/pdbsum/2mp9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mp9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/AFP_CENMR AFP_CENMR] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Antimicrobial peptides form part of the first line of defense against pathogens for many organisms. Current treatments for fungal infections are limited by drug toxicity and pathogen resistance. Cm-p5 (SRSELIVHQRLF), a peptide derived from the marine mollusk Cenchritis muricatus peptide Cm-p1, has a significantly increased fungistatic activity against pathogenic Candida albicans (minimal inhibitory concentration, 10 microg/ml; EC50, 1.146 microg/ml) while exhibiting low toxic effects against a cultured mammalian cell line. Cm-p5 as characterized by circular dichroism and nuclear magnetic resonance revealed an alpha-helical structure in membrane-mimetic conditions and a tendency to random coil folding in aqueous solutions. Additional studies modeling Cm-p5 binding to a phosphatidylserine bilayer in silico and isothermal titration calorimetry using lipid monophases demonstrated that Cm-p5 has a high affinity for the phospholipids of fungal membranes (phosphatidylserine and phosphatidylethanolamine), only moderate interactions with a mammalian membrane phospholipid, low interaction with ergosterol, and no interaction with chitin. Adhesion of Cm-p5 to living C. albicans cells was confirmed by fluorescence microscopy with FITC-labeled peptide. In a systemic candidiasis model in mice, intraperitoneal administration of Cm-p5 was unable to control the fungal kidney burden, although its low amphiphaticity could be modified to generate new derivatives with improved fungicidal activity and stability.-Lopez-Abarrategui, C., McBeth, C., Mandal, S. M., Sun, Z. J., Heffron, G., Alba-Menendez, A., Migliolo, L., Reyes-Acosta, O., Garcia-Villarino, M., Nolasco, D. O., Falcao, R., Cherobim, M. D., Dias, S. C., Brandt, W., Wessjohann, L., Starnbach, M., Franco, O. L., Otero-Gonzalez, A. J. Cm-p5: an antifungal hydrophilic peptide derived from the coastal mollusk Cenchritis muricatus (Gastropoda: Littorinidae). | |||
Cm-p5: an antifungal hydrophilic peptide derived from the coastal mollusk Cenchritis muricatus (Gastropoda: Littorinidae).,Lopez-Abarrategui C, McBeth C, Mandal SM, Sun ZJ, Heffron G, Alba-Menendez A, Migliolo L, Reyes-Acosta O, Garcia-Villarino M, Nolasco DO, Falcao R, Cherobim MD, Dias SC, Brandt W, Wessjohann L, Starnbach M, Franco OL, Otero-Gonzalez AJ FASEB J. 2015 Aug;29(8):3315-25. doi: 10.1096/fj.14-269860. Epub 2015 Apr 28. PMID:25921828<ref>PMID:25921828</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2mp9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cenchritis muricatus]] | |||
[[Category: Large Structures]] | |||
[[Category: Heffron G]] | |||
[[Category: Mcbeth C]] | |||
[[Category: Otero-Gonzales AJ]] | |||
[[Category: Starnbach MN]] | |||
[[Category: Sun ZJ]] | |||
[[Category: Wagner G]] | |||
