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[[Image:1cvx.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF POLYAMIDE DIMER (IMPYHPPYBETADP)2 BOUND TO B-DNA DECAMER CCAGATCTGG==
|PDB= 1cvx |SIZE=350|CAPTION= <scene name='initialview01'>1cvx</scene>, resolution 2.27&Aring;
<StructureSection load='1cvx' size='340' side='right'caption='[[1cvx]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=HP2:HYDROXYPYRROLE-IMIDAZOLE-PYRROLE POLYAMIDE'>HP2</scene>
<table><tr><td colspan='2'>[[1cvx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CVX FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HP2:HYDROXYPYRROLE-IMIDAZOLE-PYRROLE+POLYAMIDE'>HP2</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cvx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cvx OCA], [https://pdbe.org/1cvx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cvx RCSB], [https://www.ebi.ac.uk/pdbsum/1cvx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cvx ProSAT]</span></td></tr>
 
</table>
'''CRYSTAL STRUCTURE OF POLYAMIDE DIMER (IMPYHPPYBETADP)2 BOUND TO B-DNA DECAMER CCAGATCTGG'''
__TOC__
 
</StructureSection>
 
[[Category: Large Structures]]
==Overview==
[[Category: Baird EE]]
Synthetic polyamides composed of three types of aromatic amino acids, N-methylimidazole (Im), N-methylpyrrole (Py) and N-methyl-3-hydroxypyrrole (Hp) bind specific DNA sequences as antiparallel dimers in the minor groove. The side-by-side pairings of aromatic rings in the dimer afford a general recognition code that allows all four base-pairs to be distinguished. To examine the structural consequences of changing the DNA sequence context on T.A recognition by Hp/Py pairs in the minor groove, crystal structures of polyamide dimers (ImPyHpPy)(2) and the pyrrole counterpart (ImPyPyPy)(2) bound to the six base-pair target site 5'-AGATCT-3' in a ten base-pair oligonucleotide have been determined to a resolution of 2.27 and 2.15 A, respectively. The structures demonstrate that the principles of Hp/Py recognition of T.A are consistent between different sequence contexts. However, a general structural explanation for the non-additive reduction in binding affinity due to introduction of the hydroxyl group is less clear. Comparison with other polyamide-DNA cocrystal structures reveals structural themes and differences that may relate to sequence preference.
[[Category: Bremer RE]]
 
[[Category: Dervan PB]]
==About this Structure==
[[Category: Kielkopf CL]]
1CVX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CVX OCA].  
[[Category: Rees DC]]
 
[[Category: White S]]
==Reference==
Structural effects of DNA sequence on T.A recognition by hydroxypyrrole/pyrrole pairs in the minor groove., Kielkopf CL, Bremer RE, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC, J Mol Biol. 2000 Jan 21;295(3):557-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10623546 10623546]
[[Category: Protein complex]]
[[Category: Baird, E E.]]
[[Category: Bremer, R E.]]
[[Category: Dervan, P B.]]
[[Category: Kielkopf, C L.]]
[[Category: Rees, D C.]]
[[Category: White, S.]]
[[Category: HP2]]
[[Category: double drug in minor groove]]
[[Category: minor groove recognition]]
[[Category: polyamide]]
[[Category: ta recognition]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:29:33 2008''

Latest revision as of 06:46, 7 February 2024

CRYSTAL STRUCTURE OF POLYAMIDE DIMER (IMPYHPPYBETADP)2 BOUND TO B-DNA DECAMER CCAGATCTGG

1cvx, resolution 2.27Å

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