Barnase: Difference between revisions

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<StructureSection load='1brn' size='350' side='right' scene='44/442736/Cv/2' caption='Barnase complex with tetranucleotide (PDB code [[1brn]])'>


{{STRUCTURE_1bni|  PDB=1bni  | SIZE=400| SCENE=Barnase/Cv/1 |right|CAPTION=3 monomers of barnase [[1bni]] }}
== Function ==


'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial ribonuclease synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.
'''Barnase''' (BA, BActerial RiboNucleASE) is a bacterial [[ribonuclease]] synthesized and secreted by the bacterium ''Bacillus amyloliquefaciens''. <ref>PMID:8110767</ref> The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.  


__NOTOC__
== Relevance ==


== 3D Structures of Barnase ==
BA structure was used extensively for studying protein folding.


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
== Structural highlights ==


[[3kch]], [[2f4y]] – BA cross-linked<br />
BA hydrolyzes RNA at GpN sites where the <scene name='44/442736/Cv/4'>active site positively charged Lys and Arg coordinate the RNA phosphate group</scene> (Lys and Arg are in magenta).
[[2f56]] - BA cross-linked + urea<br />
[[2f5w]] - BA cross-linked + thiourea<br />
[[2f5m]] - BA cross-linked + Bromo-ethanol<br />
[[1fw7]], [[1bnr]] – BA - NMR<br />
[[2kf3]] – BA (mutant) – NMR<br />
[[2kf4]], [[1b2x]], [[1a2p]], [[1bni]], [[1bnj]] – BA<br />
[[1yvs]] – BA domain swapped<br />
[[1b20]], [[1b21]], [[1b2z]], [[1bne]], [[1bnf]], [[1bng]], [[1brh]], [[1bri]], [[1brj]], [[1brk]], [[1bns]], [[1brg]], [[1bsa]], [[1bsb]], [[1bsc]], [[1bsd]], [[1bse]], [[1ban]], [[1bao]] – BA (mutant)<br />
[[2kf5]], [[2kf6]], [[1brn]], [[1rnb]] – BA + polynucleotide


===BA + BST===
== 3D Structures of Barnase ==
 
[[Barnase 3D structures]]
[[3da7]] - BA (mutant) + BST<br />
[[2za4]], [[1brs]], [[1bgs]] – BA + BST<br />
[[1x1u]], [[1x1w]], [[1x1x]], [[1x1y]], [[1b27]], [[1b2u]] – BA + BST (mutant)<br />
[[1b3s]], [[1b2s]] - BA (mutant) + BST (mutant)<br />


[[2c4b]] – BA/MCOEETI (mutant)
</StructureSection>


== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]
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