4r84: Difference between revisions
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New page: '''Unreleased structure''' The entry 4r84 is ON HOLD until Paper Publication Authors: Fisher, A.J., Chen, X., Li, Y., Huynh, N. Description: Crystal structure of Sialyltransferase from... |
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==Crystal structure of Sialyltransferase from Photobacterium damsela with CMP-3F(a)Neu5Ac bound== | |||
<StructureSection load='4r84' size='340' side='right'caption='[[4r84]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4r84]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Photobacterium_damselae Photobacterium damselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R84 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CSF:CYTIDINE-5-MONOPHOSPHATE-3-FLUORO-N-ACETYL-NEURAMINIC+ACID'>CSF</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r84 OCA], [https://pdbe.org/4r84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r84 RCSB], [https://www.ebi.ac.uk/pdbsum/4r84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r84 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/O66375_9GAMM O66375_9GAMM] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae alpha2-6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of alpha2-6-linked sialosides. Here we report three crystal structures of this enzyme. Two structures with and without a donor substrate analog CMP-3F(a)Neu5Ac contain an immunoglobulin (Ig)-like domain and adopt the GT-B sialyltransferase fold. The binary structure reveals a non-productive pre-Michaelis complex, which are caused by crystal lattice contacts that prevent the large conformational changes. The third structure lacks the Ig-domain. Comparison of the three structures reveals small inherent flexibility between the two Rossmann-like domains of the GT-B fold. | |||
Crystal structures of sialyltransferase from Photobacterium damselae.,Huynh N, Li Y, Yu H, Huang S, Lau K, Chen X, Fisher AJ FEBS Lett. 2014 Dec 20;588(24):4720-9. doi: 10.1016/j.febslet.2014.11.003. Epub, 2014 Nov 15. PMID:25451227<ref>PMID:25451227</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4r84" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Photobacterium damselae]] | |||
[[Category: Chen X]] | |||
[[Category: Fisher AJ]] | |||
[[Category: Huynh N]] | |||
[[Category: Li Y]] | |||
Latest revision as of 17:45, 20 September 2023
Crystal structure of Sialyltransferase from Photobacterium damsela with CMP-3F(a)Neu5Ac bound
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