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[[Image:2daa.gif|left|200px]]<br />
<applet load="2daa" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2daa, resolution 2.1&Aring;" />
'''CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE INACTIVATED BY D-CYCLOSERINE'''<br />


==About this Structure==
==CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE INACTIVATED BY D-CYCLOSERINE==
2DAA is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_sp. Bacillus sp.]] with DCS as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/D-amino-acid_transaminase D-amino-acid transaminase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.6.1.21 2.6.1.21]]. Structure known Active Sites: ASA and ASB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2DAA OCA]].
<StructureSection load='2daa' size='340' side='right'caption='[[2daa]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
[[Category: Bacillus sp.]]
== Structural highlights ==
[[Category: D-amino-acid transaminase]]
<table><tr><td colspan='2'>[[2daa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._(in:_Bacteria) Bacillus sp. (in: Bacteria)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DAA FirstGlance]. <br>
[[Category: Single protein]]
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
[[Category: Chipman, D.M.]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DCS:D-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-N,O-CYCLOSERYLAMIDE'>DCS</scene></td></tr>
[[Category: Peisach, D.]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2daa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2daa OCA], [https://pdbe.org/2daa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2daa RCSB], [https://www.ebi.ac.uk/pdbsum/2daa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2daa ProSAT]</span></td></tr>
[[Category: Ringe, D.]]
</table>
[[Category: DCS]]
== Function ==
[[Category: aminotransferase]]
[https://www.uniprot.org/uniprot/DAAA_BACYM DAAA_BACYM] Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha-keto acid in the first half-reaction. The second-half reaction is the reverse of the first, transferring the amino group from the pyridoxamine to a second alpha-keto acid to form the product D-amino acid via a ping-pong mechanism. This is an important process in the formation of D-alanine and D-glutamate, which are essential bacterial cell wall components.<ref>PMID:2914916</ref> <ref>PMID:9538014</ref>
[[Category: antibiotic]]
== Evolutionary Conservation ==
[[Category: cycloserine]]
[[Image:Consurf_key_small.gif|200px|right]]
[[Category: pyridoxal phosphate]]
Check<jmol>
[[Category: pyridoxamine]]
  <jmolCheckbox>
[[Category: suicide substrate]]
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/da/2daa_consurf.spt"</scriptWhenChecked>
[[Category: transaminase]]
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
[[Category: transferase]]
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2daa ConSurf].
<div style="clear:both"></div>


''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 17:14:09 2007''
==See Also==
*[[Aminotransferase 3D structures|Aminotransferase 3D structures]]
*[[Aspartate aminotransferase 3D structures|Aspartate aminotransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Chipman DM]]
[[Category: Peisach D]]
[[Category: Ringe D]]

Latest revision as of 06:42, 9 August 2023

CRYSTALLOGRAPHIC STRUCTURE OF D-AMINO ACID AMINOTRANSFERASE INACTIVATED BY D-CYCLOSERINE

2daa, resolution 2.10Å

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