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| {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]] }}
| | <StructureSection load='1kcf' size='340' side='right' caption='Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]]' scene='' > |
| [[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The '''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. '''Tyr-recombinases''' include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). '''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. '''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>. | | == Function == |
| | [[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. |
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| | *'''Holliday junction resolvase''' (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands<ref>PMID:19442245</ref>. <br /> |
| | *'''Tyr-recombinases''' include '''Cre''' which recombines loxP sites, FLP and lambda integrase (LamInt). <br /> |
| | *'''Ser-recombinases''' (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase.<br /> |
| | *'''RadA recombinase''' promotes DNA recombination. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:19143611</ref>. <br /> |
| | *'''RadB recombinase''' is a paralogue of RadA assisting in RadA-mediated strand exchange <ref>PMID:28501701</ref>. <br /> |
| | *'''Hin-recombinase''' (HRec) is a protein of ''Salmonella''. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions<ref>PMID:15454079</ref>. |
| | *'''Tre recombinase''' recognizes a target sequence of the HIV virus resulting in excision and eradication of the provirus from infected human cells <ref>PMID:19066582</ref>. <br /> |
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| ==3D structures of resolvase== | | ==3D structures of resolvase== |
| | [[Resolvase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
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| === Holliday junction resolvase ===
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| [[2wiw]], [[2wiz]], [[2wj0]] – AfHJR+DNA – ''Archaeoglobus fulgidus''<br />
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| [[2wcw]], [[2wcz]] – AfHJR (mutant)<br />
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| [[2h8c]] – EcHJR RusA (mutant) + DNA – ''Escherichia coli''<br />
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| [[1gdt]] - EcHJR +DNA<br />
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| [[2h8e]] - EcHJR RusA (mutant)<br />
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| [[1q8r]] – EcHJR RusA<br />
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| [[1hjr]] – EcHJR RuvC<br />
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| [[4ep4]] – TtHJR RuvC – ''Thermus thermophilus''<br />
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| [[4ep5]] – TtHJR RuvC (mutant)<br />
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| [[1zp7]] – HJR RecU – ''Bacillus subtilis''<br />
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| [[1ob8]], [[1ob9]], [[1hh1]] – SsHJR – ''Sulfolobus solfataricus''<br />
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| [[2fco]] – HJR – ''Geobacillus kaustophilus''<br />
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| [[1ipi]], [[1gef]] – HJR – ''Pyrococcus furiosus''<br />
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| [[1kcf]] – HJR – fission yeast<br />
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| === Ser-recombinase ===
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| [[2rsl]] - EcGDR<br />
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| [[1hx7]] – EcGDR N-terminal – NMR<br />
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| [[1ght]], [[1res]], [[1ret]], [[1ght]] – EcGDR catalytic domain – NMR<br />
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| [[1gdr]], [[1hx7]] - EcGDR catalytic domain<br />
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| [[2gm4]], [[1zr2]], [[1zr4]], [[1gdt]] – EcGDR+DNA<br />
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| [[2gm5]] – EcGDR (mutant)<br />
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| [[3guv]] – site-specific recombinase – ''Streptococcus pneumoniae''<br />
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| [[2r0q]] – S-rec+DNA – ''Staphylococcus aureus''<br />
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| [[3lhf]] – SsS-rec<br />
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| === Tyr-recombinase ===
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| [[3etl]], [[3ew9]], [[3ewa]], [[2i1q]] – MmRadA Rec+AMPPNP+ion – ''Methanococcus maripaludis''<br />
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| [[2b21]] - MmRadA Rec+AMPPNP<br />
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| [[2zub]], [[2zuc]], [[2zud]], [[2dfl]] – SsRadA<br />
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| [[2cvf]], [[2cvh]] – RadB Rec – ''Thermococcus kodakarensis''<br />
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| [[2f1h]], [[2fpl]], [[2fpm]] - MvRec+AMPPNP+K – ''Methanococcus voltae''<br />
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| [[2f1i]] – MvRec+AMPPNP<br />
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| [[2f1j]], [[2fpk]] - MvRec+ADP<br />
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| [[1a0p]] – EcXerd Rec<br />
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| === Cre recombinase ===
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| [[3mgv]], [[3c28]], [[3c29]], [[2hof]], [[2hoi]], [[1xns]], [[1xo0]], [[1pvp]], [[1pvq]], [[1pvr]], [[1nzb]], [[1ouq]], [[1q3u]], [[1q3v]], [[1ma7]], [[1kbu]], [[1drg]], [[1f44]], [[2crx]], [[3crx]], [[4crx]], [[5crx]], [[1crx]] – EpCre+DNA – Enterobacteria phage PI<br />
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| === Integrase ===
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| [[2a3v]] – Rec INTI4+DNA – ''Vibrio cholerae''<br />
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| [[3bvp]] – Integrase N-terminal – Lactococcus phage<br />
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| [[2oxo]] – LamInt binding domain – phage<br />
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| [[1z19]] – EpLamInt core binding +catalytic domain+DNA<br />
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| [[1z1b]], [[1z1g]], [[1p7d]] – EpLamInt+DNA<br />
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| [[1kjk]] – EpLamInt N-terminal – NMR<br />
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| [[1ae9]] – EpLamInt catalytic core<br />
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| [[1p4e]], [[1m6x]] – yFLP (mutant)+DNA – yeast<br />
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| [[1flo]] - yFLP +DNA<br />
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| [[1aih]] – HP1 integrase catalytic domain - bacteriophage<br />
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| [[3nrw]] – INT/site-specific recombinase N-terminal – ''Haloarcula marismortui''<br />
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| [[3jtz]] – INT arm-type binding domain – ''Yersinia pestis''<br />
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| [[3ju0]] – HPI INT arm-type binding domain – ''Pectobacterium atrosepticum''<br />
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| [[2kkp]] – INT SAM-like domain – ''Moorella thermoacetica'' – NMR<br />
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| [[2kkv]] – INT fragment – ''Salmonella enterica'' – NMR<br />
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| [[2khq]] - INT fragment – ''Staphylococcus saprophyticus'' – NMR<br />
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| [[2wcc]] – λINT DBD+ DNA – Enterobacteria phage λ - NMR<br />
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| [[2khv]], [[2kj5]] – INT residues 102-199 – ''Nitrosospira multiformis'' – NMR<br />
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| ===Recombinase A (RecA)===
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| [[Recombinase A]]
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| ==References== | | ==References== |
