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[[Image:1ei6.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF PHOSPHONOACETATE HYDROLASE COMPLEXED WITH PHOSPHONOFORMATE==
|PDB= 1ei6 |SIZE=350|CAPTION= <scene name='initialview01'>1ei6</scene>, resolution 2.1&Aring;
<StructureSection load='1ei6' size='340' side='right'caption='[[1ei6]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene> and <scene name='pdbligand=PPF:PHOSPHONOFORMIC ACID'>PPF</scene>
<table><tr><td colspan='2'>[[1ei6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EI6 FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphonoacetate_hydrolase Phosphonoacetate hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.11.1.2 3.11.1.2]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPF:PHOSPHONOFORMIC+ACID'>PPF</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ei6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ei6 OCA], [https://pdbe.org/1ei6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ei6 RCSB], [https://www.ebi.ac.uk/pdbsum/1ei6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ei6 ProSAT]</span></td></tr>
 
</table>
'''CRYSTAL STRUCTURE OF PHOSPHONOACETATE HYDROLASE COMPLEXED WITH PHOSPHONOFORMATE'''
== Function ==
 
[https://www.uniprot.org/uniprot/PHNHY_PSEFL PHNHY_PSEFL] Specifically hydrolyzes phosphonoacetate. Does not have activity on other organophosphonates or acetates.<ref>PMID:8288524</ref>
 
== Evolutionary Conservation ==
==About this Structure==
[[Image:Consurf_key_small.gif|200px|right]]
1EI6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EI6 OCA].  
Check<jmol>
[[Category: Phosphonoacetate hydrolase]]
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ei/1ei6_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ei6 ConSurf].
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas fluorescens]]
[[Category: Pseudomonas fluorescens]]
[[Category: Single protein]]
[[Category: Benning MM]]
[[Category: Benning, M M.]]
[[Category: Dunaway-Mariano D]]
[[Category: Dunaway-Mariano, D.]]
[[Category: Holden HM]]
[[Category: Holden, H M.]]
[[Category: Kim AD]]
[[Category: Kim, A D.]]
[[Category: PPF]]
[[Category: TLA]]
[[Category: ZN]]
[[Category: crystal structure]]
[[Category: hydrolase]]
[[Category: phosphonoacetate]]
[[Category: zinc]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 10:55:55 2008''

Latest revision as of 07:03, 7 February 2024

CRYSTAL STRUCTURE OF PHOSPHONOACETATE HYDROLASE COMPLEXED WITH PHOSPHONOFORMATE

1ei6, resolution 2.10Å

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