Transketolase: Difference between revisions

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{{STRUCTURE_1tka| PDB=1tka  | SIZE=400| SCENE= |right|CAPTION=Transketolase dimer complex with deazo-thiamine diphosphate and Ca+2 ion (green) [[1tka]] }}
<StructureSection load='' size='350' side='right' caption='E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green) (PDB code [[2r8o]]).' scene='46/466534/Cv/1'>
== Function ==
'''Transketolase''' (TKT) catalyzes two opposite reactions. The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the [[Calvin cycle]]<ref>PMID:8369276</ref>. '''Split-gene TKT''' is found in the hyperthermophilic bacterium ''Carboxydothermus hydrogenoformans'' and is reconstituted from 2 separate polypeptide chains<ref>PMID:33193259</ref>.


'''Transketolase''' (TKT) catalyzes two opposite reactions.  The synthesis of sedoheptulose-7-P in the pentose phosphate pathway using thiamine diphosphate (TPP) as co-factor; and the conversion of sedoheptulose-7-P and glyceraldehyde-3-P to aldose and ketose in the Calvin cycle.
== Structural highlights ==


{{TOC limit|limit=2}}
The <scene name='46/466534/Cv/7'>active site of TKT contains a ligand-cofactor adduct</scene>.<ref>PMID:17914867</ref> Water molecules are shown as red spheres. <scene name='46/466534/Cv/8'>Ca coordination site</scene>.


== 3D Structures of transketolase ==
== 3D Structures of transketolase ==


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
[[Transketolase 3D structures]]
 
</StructureSection>
=== Transketolase ===
 
[[1trk]] – yTKT – yeast
 
[[1ay0]] – yTKT (mutant)
 
[[2e6k]] – TKT – ''Thermus thermophilus''
 
[[3hyl]] – BaTKT – ''Bacillus anthracis''
 
[[3kom]] – TKT (mutant) – ''Francisella tularensis''
 
[[3l84]] – CjTKT – ''Campylobacter jejuni''<br />
[[3rim]] – TKT – ''Mycobacterium tuberculosis''<br />
[[3uk1]] – BtTKT – ''Burkholderia thailandensis''
 
===Transketolase binary complexes===
 
[[3m49]] – BaTKT + TPP
 
[[3mos]], [[3ooy]] – hTKT + TPP - human<br />
[[4kxy]] - hTKT + TPP analog<br />
[[1tka]], [[1tkb]], [[1tkc]] – yTKT + TPP analog
 
[[1gpu]] - yTKT + TPP derivative
 
[[1itz]] - TKT + TPP – maize
 
[[1qgd]] - EcTKT + TPP – ''Escherichia coli''
 
[[1r9j]] - TKT + TPP – ''Leishmania mexicana''
 
===Transketolase ternary complexes===
 
[[3m7i]], [[3m6l]] – CjTKT + TPP + ribose 5-P
 
[[3m34]] - CjTKT + TPP + Ca
 
[[1ngs]] - yTKT + TPP + Ca + erythrose-4-P
 
[[2r5n]] – EcTKT + TPP + ribose 5-P<br />
[[3upt]] - BtTKT + TPP + ribose 5-P<br />
[[2r8o]] - EcTKT + TPP + xylulose 5-P
 
[[2r8p]] - EcTKT + TPP + fructose 6-P<br />
[[4kxu]] - hTKT + TPP + sorbitol 6-P<br />
[[4kxv]], [[4kxw]] - hTKT + TPP + xylitol 5-P<br />
[[4kxx]] - hTKT + TPP + seduheptulose 7-P<br />




== References ==
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 13:54, 4 January 2023

E. coli transketolase 1 dimer complex with xylulose-5-phosphate-thiamine diphosphate adduct, ethylene glycol and Ca+2 ion (green) (PDB code 2r8o).

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References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky, Joel L. Sussman

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