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[[Image:1f08.gif|left|200px]]


{{Structure
==CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS==
|PDB= 1f08 |SIZE=350|CAPTION= <scene name='initialview01'>1f08</scene>, resolution 1.90&Aring;
<StructureSection load='1f08' size='340' side='right'caption='[[1f08]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=BR:BROMIDE ION'>BR</scene>
<table><tr><td colspan='2'>[[1f08]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F08 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f08 OCA], [https://pdbe.org/1f08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f08 RCSB], [https://www.ebi.ac.uk/pdbsum/1f08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f08 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VE1_BPV1 VE1_BPV1] ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f0/1f08_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1f08 ConSurf].
<div style="clear:both"></div>


'''CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS'''
==See Also==
 
*[[Replication protein E1|Replication protein E1]]
 
__TOC__
==Overview==
</StructureSection>
Papillomaviral infection causes both benign and malignant lesions and is a necessary cause of cervical carcinoma. Replication of this virus requires the replication initiation proteins E1 and E2, which bind cooperatively at the origin of replication (ori) as an (E1)2-(E2)2-DNA complex. This is a precursor to larger E1 complexes that distort and unwind the ori. We present the crystal structure of the E1 DNA binding domain refined to 1.9 A resolution. Residues critical for DNA binding are located on an extended loop and an alpha helix. We identify the E1 dimerization surface by selective mutations at an E1/E1 interface observed in the crystal and propose a model for the (E1)2-DNA complex. These and other observations suggest how the E1 DNA binding domain orchestrates assembly of the hexameric helicase on the ori.
 
==About this Structure==
1F08 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bovine_papillomavirus Bovine papillomavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F08 OCA].
 
==Reference==
Crystal structure of the DNA binding domain of the replication initiation protein E1 from papillomavirus., Enemark EJ, Chen G, Vaughn DE, Stenlund A, Joshua-Tor L, Mol Cell. 2000 Jul;6(1):149-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10949036 10949036]
[[Category: Bovine papillomavirus]]
[[Category: Bovine papillomavirus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chen, G.]]
[[Category: Chen G]]
[[Category: Enemark, E J.]]
[[Category: Enemark EJ]]
[[Category: Joshua-Tor, L.]]
[[Category: Joshua-Tor L]]
[[Category: Stenlund, A.]]
[[Category: Stenlund A]]
[[Category: Vaughn, D E.]]
[[Category: Vaughn DE]]
[[Category: BR]]
[[Category: dna-binding domain]]
[[Category: helicase]]
[[Category: initiator protein]]
[[Category: papillomavirus]]
[[Category: replication]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:02:37 2008''

Latest revision as of 07:09, 7 February 2024

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF THE REPLICATION INITIATION PROTEIN E1 FROM PAPILLOMAVIRUS

1f08, resolution 1.90Å

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