1fqi: Difference between revisions

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[[Image:1fqi.gif|left|200px]]


{{Structure
==RGS9 RGS DOMAIN==
|PDB= 1fqi |SIZE=350|CAPTION= <scene name='initialview01'>1fqi</scene>, resolution 1.94&Aring;
<StructureSection load='1fqi' size='340' side='right'caption='[[1fqi]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1fqi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1FQI FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1fqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fqi OCA], [https://pdbe.org/1fqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1fqi RCSB], [https://www.ebi.ac.uk/pdbsum/1fqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1fqi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RGS9_BOVIN RGS9_BOVIN] Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fq/1fqi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1fqi ConSurf].
<div style="clear:both"></div>


'''RGS9 RGS DOMAIN'''
==See Also==
 
*[[Regulator of G-protein signaling 3D structures|Regulator of G-protein signaling 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
A multitude of heptahelical receptors use heterotrimeric G proteins to transduce signals to specific effector target molecules. The G protein transducin, Gt, couples photon-activated rhodopsin with the effector cyclic GMP phosophodiesterase (PDE) in the vertebrate phototransduction cascade. The interactions of the Gt alpha-subunit (alpha(t)) with the inhibitory PDE gamma-subunit (PDEgamma) are central to effector activation, and also enhance visual recovery in cooperation with the GTPase-activating protein regulator of G-protein signalling (RGS)-9 (refs 1-3). Here we describe the crystal structure at 2.0 A of rod transducin alpha x GDP x AlF4- in complex with the effector molecule PDEgamma and the GTPase-activating protein RGS9. In addition, we present the independently solved crystal structures of the RGS9 RGS domain both alone and in complex with alpha(t/i1) x GDP x AlF4-. These structures reveal insights into effector activation, synergistic GTPase acceleration, RGS9 specificity and RGS activity. Effector binding to a nucleotide-dependent site on alpha(t) sequesters PDEgamma residues implicated in PDE inhibition, and potentiates recruitment of RGS9 for hydrolytic transition state stabilization and concomitant signal termination.
 
==About this Structure==
1FQI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FQI OCA].
 
==Reference==
Structural determinants for regulation of phosphodiesterase by a G protein at 2.0 A., Slep KC, Kercher MA, He W, Cowan CW, Wensel TG, Sigler PB, Nature. 2001 Feb 22;409(6823):1071-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11234020 11234020]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Cowan, C W.]]
[[Category: Cowan CW]]
[[Category: He, W.]]
[[Category: He W]]
[[Category: Kercher, M A.]]
[[Category: Kercher MA]]
[[Category: Sigler, P B.]]
[[Category: Sigler PB]]
[[Category: Slep, K C.]]
[[Category: Slep KC]]
[[Category: Wensel, T G.]]
[[Category: Wensel TG]]
[[Category: gap]]
[[Category: phototransduction]]
[[Category: rg]]
[[Category: rgs9]]
[[Category: rod]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:12:39 2008''

Latest revision as of 11:15, 27 March 2024

RGS9 RGS DOMAIN

1fqi, resolution 1.94Å

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