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==Crystal structure of pectin methylesterase in complex with hexasaccharide VI==
==Crystal structure of pectin methylesterase in complex with hexasaccharide VI==
<StructureSection load='2ntp' size='340' side='right' caption='[[2ntp]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
<StructureSection load='2ntp' size='340' side='right'caption='[[2ntp]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2ntp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NTP FirstGlance]. <br>
<table><tr><td colspan='2'>[[2ntp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NTP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NTP FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene>, <scene name='pdbligand=SHB:METHYL+BETA-D-GALACTOPYRANURONATE'>SHB</scene><br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1qjv|1qjv]], [[2nsp|2nsp]], [[2nst|2nst]], [[2nt6|2nt6]], [[2nt9|2nt9]], [[2ntb|2ntb]], [[2ntq|2ntq]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADA:ALPHA-D-GALACTOPYRANURONIC+ACID'>ADA</scene>, <scene name='pdbligand=M8C:METHYL+ALPHA-D-GALACTOPYRANURONATE'>M8C</scene>, <scene name='pdbligand=SHB:METHYL+BETA-D-GALACTOPYRANURONATE'>SHB</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pemA, pem ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=556 Erwinia chrysanthemi])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ntp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntp OCA], [https://pdbe.org/2ntp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ntp RCSB], [https://www.ebi.ac.uk/pdbsum/2ntp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ntp ProSAT]</span></td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pectinesterase Pectinesterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.11 3.1.1.11] </span></td></tr>
</table>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ntp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ntp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2ntp RCSB], [http://www.ebi.ac.uk/pdbsum/2ntp PDBsum]</span></td></tr>
== Function ==
<table>
[https://www.uniprot.org/uniprot/PMEA_DICD3 PMEA_DICD3] Involved in maceration and soft-rotting of plant tissue.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntp_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/2ntp_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ntp ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 2ntp" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Methylesterase|Methylesterase]]
*[[Methylesterase 3D structures|Methylesterase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Erwinia chrysanthemi]]
[[Category: Dickeya dadantii 3937]]
[[Category: Pectinesterase]]
[[Category: Large Structures]]
[[Category: Brocklehurst, K.]]
[[Category: Brocklehurst K]]
[[Category: Fries, M.]]
[[Category: Fries M]]
[[Category: Pickersgill, R W.]]
[[Category: Pickersgill RW]]
[[Category: Shevchik, V E.]]
[[Category: Shevchik VE]]
[[Category: Hydrolase]]
[[Category: Pme hexasaccharide complex]]

Latest revision as of 07:43, 9 October 2024

Crystal structure of pectin methylesterase in complex with hexasaccharide VI

2ntp, resolution 1.70Å

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